Article: HCN1 channels control resting and active integrative properties of stellate cells from layer II of the entorhinal cortex.

The membrane potential (V_m) along with input resistance determined from positive (IR+ve) and negative (IR−ve) current steps were first estimated in control conditions and then in the presence of 10 μm ZD7288 without any offset current (ZD7288) and also with the membrane potential returned to its control value by injection of a constant offset current (ZD7288 offset). ANOVA demonstrated significant effects of both genotype and ZD7288 on V_m (p = 0.013 and p = 1e-7), IR−ve (p = 0.004 and p = 0.018), IR+ve (p = 0.016 and p = 0.037), and sag (p = 1e-6 and p = 1e-7). Post hoc analysis (t test) indicates that, in control conditions, there are significant differences between stellate cells from HCN1^+/+ and HCN1^−/− mice in their V_m (p = 0.0038), IR−ve (p = 0.0066), IR+ve (p = 0.017), and sag (p = 0.0005). In contrast, in the presence of ZD7288, there are no longer any significant differences between HCN1^+/+ and HCN1^−/− mice in V_m (p = 0.51), IR−ve (p = 0.58), or IR+ve (p = 0.52).