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Article: The FGF14(F145S) mutation disrupts the interaction of FGF14 with voltage-gated Na+ channels and impairs neuronal excitability.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Laezza F; Gerber BR; Lou JY; Kozel MA; Hartman H; Craig AM; Ornitz DM; Nerbonne JM J. Neurosci., 2007
Table 2.
Passive and active membrane properties of rat hippocampal neurons
| Cm (pF) | Rin (GΩ) | Vm (mV) | Ithreshold (pA) | Vthreshold (mV) | APA (mV) | APD50 (ms) | |
|---|---|---|---|---|---|---|---|
| GFP (n = 30) | 44 ± 3 | 0.7 ± 0.1 | −56 ± 1 | 38 ± 6 | −28 ± 0.9 | 81 ± 2 | 3.7 ± 0.3 |
| FGF14 (n = 13) | 53 ± 4 | 0.7 ± 0.1 | −55 ± 1 | 34 ± 6 | −28 ± 1.4 | 78 ± 3 | 4.4 ± 0.2 |
| FGF14F145S (n = 16) | 49 ± 5 | 0.7 ± 0.1 | −58 ± 1 | 62 ± 7*** | −26 ± 1.3 | 76 ± 4 | 4.4 ± 0.4 |
| GFP (n = 6) | 52 ± 6 | 0.7 ± 0.1 | −57 ± 2 | 24 ± 4 | −30 ± 0.5 | 89 ± 8 | 3.8 ± 1.2 |
| +1 nm TTX (n = 4) | 56 ± 8* | −30 ± 0.9 | 87 ± 5 | 3.1 ± 0.3 | |||
| +5 nm TTX (n = 4) | 88 ± 6** | −25 ± 3.7 | 87 ± 1 | 4.1 ± 0.5 |
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All values are means ± SEM. APA, Action potential amplitude; APD50, action potential duration at 50% repolarization.
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↵***p < 0.001, significantly different from the values measured in GFP- or FGF14-expressing cells
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↵**p < 0.005 and
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↵*p < 0.01, significantly different from those measured in control recordings in the absence of TTX.
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
|---|---|---|---|---|---|
| Other | cultured hippocampus neurons | cell capacitance (pF) | 44.0 ± 3.0 (30) | 44.0 (pF) | Data Table |
| Other | cultured hippocampus neurons | input resistance (GΩ) | 0.7 ± 0.1 (30) | 700.0 (MΩ) | Data Table |
| Other | cultured hippocampus neurons | resting membrane potential (mV) | -56.0 ± 1.0 (30) | -56.0 (mV) | Data Table |
| Other | cultured hippocampus neurons | rheobase (pA) | 38.0 ± 6.0 (30) | 38.0 (pA) | Data Table |
| Other | cultured hippocampus neurons | spike threshold (mV) | -28.0 ± 0.9 (30) | -28.0 (mV) | Data Table |
| Other | cultured hippocampus neurons | spike amplitude (mV) | 81.0 ± 2.0 (30) | 81.0 (mV) | Data Table |
| Other | cultured hippocampus neurons | spike half-width (ms) | 3.7 ± 0.3 (30) | 3.7 (ms) | Data Table |