Article: Effects of variations in hippocampal slice preparation protocol on the electrophysiological stability, epileptogenicity and graded hypoxia responses of CA1 neurons.
Table 3Summary of the effects of method of dissection and incubation temperature on properties of orthodromically evoked field potentials
28–30°C
19–21°C
Cold-shock
Orthodromic PS (mV)
Vibratome
4.63±0.98 (20)
3.80±1.46 (22)a
3.50±1.02 (18)a
Chopper
4.36±1.44 (38)
4.62±1.56 (15)
3.26±0.96 (40)b
Orthodromic field (# PS's)
Vibratome
1.05±0.22 (20)
1.45±0.67 (22)
3.11±1.13 (18)b
Chopper
1.26±0.45 (38)
1.27±0.46 (15)
3.55±1.32 (40)b
The errors represent standard deviation. aSignificant difference with respect to data collected from slices incubated at 28–30°C; bsignificant difference with respect to data collected from slices incubated at 28–30°C and 19–21°C.
