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Article: An in vitro protocol for recording from spinal motoneurons of adult rats.

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Carp JS; Tennissen AM; Mongeluzi DL; Dudek CJ; Chen XY; Wolpaw JR
J. Neurophysiol., 2008



TABLE 2.Comparison of rat hindlimb motoneuron properties recorded in vitro and in vivo


Property In Vitro

In Vivo

Fast Slow Fast Slow

Resting potential, mV –68 ± 11 –63 ± 14 –71 ± 6 –64 ± 6
AP amplitude, mV 79.9 ± 8.9 75.7 ± 8.6 76.0 ± 11.5 82.6 ± 12.2
Input resistance, mΩ 4.0 ± 2.1** 7.7 ± 4.8 1.8 ± 0.6 2.7 ± 1.2
AHP half-time, ms 14 ± 3*** 27 ± 4 14 ± 2 27 ± 7
AHP amplitude, mV –2.4 ± 1.2* –4.5 ± 2.8 –1.5 ± 1.1 –2.7 ± 1.2
Rheobase, nA 4.9 ± 3.2* 2.8 ± 1.5 9.8 ± 3.0 5.9 ± 4.7
Voltage threshold, mV –51 ± 12 –46 ± 9 –52 ± 7 –46 ± 12
Ramp onset firing rate, pps 16.7 ± 6.6** 9.2 ± 1.1 26.5 ± 7.7 16.7 ± 7.2
Ramp offset firing rate, pps 12.5 ± 3.6* 8.8 ± 2.6 24.7 ± 6.8 14.1 ± 5.6
Ramp onset firing current, nA 7.9 ± 4.6** 2.8 ± 1.8 26.5 ± 7.7 16.7 ± 7.2
Ramp offset firing current, nA 8.2 ± 4.9** 2.5 ± 1.6 10.9 ± 3.1 7 ± 4
Offset – onset current, nA 0.3 ± 0.8*** –0.3 ± 0.2 –0.2 ± 0.6 –0.4 ± 0.3
Number of motoneurons 15–26 6–10 21 7

Values are means ± SD. In vivo data are from Table 1 of Button et al. (2006), except for the action potential (AP) amplitude data, which are calculated from 216 motoneurons pooled from Table 1 of Beaumont and Gardiner (2003) and Table 1 of Cormery et al. (2005). Fast motoneurons are defined by AHP half-time <20 ms; asterisks indicate significant differences from slow motoneurons at

* P < 0.05,

** P < 0.01, and

*** P < 0.001. Slow motoneurons are defined by AHP half-time ≥20 ms. With respect to rheobase, the current threshold for AP initiation is during 40-ms depolarizing pulse. The instantaneous firing rate (ramp onset) from the first interspike interval (ISI) is that during the ascending current ramp. The instantaneous firing rate (ramp offset) from the last ISI is that during the descending current ramp. Ramp onset current is that during the ascending ramp at which firing begins. Ramp offset current is that during the descending ramp at which firing ends: Offset – onset current is the ramp current at firing offset minus the ramp current at firing onset.


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