Table 2
Morphological properties of pyramidal neurons in layer 2-6 (L2-L6) of the rat prefrontal cortex
| Layer | 2 | 3 | 5 | 5/6 | 6 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subclass | L2 | L3 RS | L3 broad Ad | L3 bursting | L3 slender Ad | L5 slender LR | L5 slender HR | L5 RS | L5 wide | L6 tall | L6 small | L6 short |
| n | 11 | 7 | 4 | 6 | 3 | 30 | 11 | 18 | 4 | 5 | 5 | 5 |
| Location within cortex | 19 ± 2% | 22 ± 2% | 26 ± 1% | 31 ± 2% | 32 ± 3% | 48 ± 2% | 53 ± 4% | 52 ± 2% | 64 ± 9% | 77 ± 1% | 77 ± 4% | 85 ± 5% |
| Vertical span apical dendrite (ending layer) | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.3 ± 0.2 | 1.0 ± 0.0 | 1.5 ± 0.5 | 1.0 ± 0.0 | 5.2 ± 0.2*/** | 4.6 ± 0.7*/** |
| Field span apical dendrite | 339 ± 20 | 259 ± 13 | 312 ± 14 | 220 ± 17 | 228 ± 14 | 290 ± 17 | 295 ± 30 | 482 ± 24*/** | 246 ± 83 | 295 ± 27 | 147 ± 10 | 361 ± 127 |
| Field span apical tuft | 339 ± 20 | 259 ± 13 | 312 ± 14 | 193 ± 20 | 228 ± 14 | 180 ± 17 | 228 ± 36 | 480 ± 25*/** | 246 ± 83 | 121 ± 48 | 60 ± 19 | 327 ± 143 |
| Field span basal dendrite | 198 ± 9 | 249 ± 14 | 270 ± 19 | 276 ± 13 | 253 ± 26 | 399 ± 10* | 324 ± 21 | 325 ± 9 | 884 ± 81** | 215 ± 8 | 181 ± 8 | 300 ± 10 |
| Ratio field span tuft/basal | 1.73 ± 0.09*/**,a | 1.05 ± 0.06 | 1.16 ± 0.03 | 0.71 ± 0.08 | 0.93 ± 0.16 | 0.46 ± 0.04 | 0.73 ± 0.12 | 1.49 ± 0.08 | 0.29 ± 0.10 | 0.58 ± 0.25 | 0.33 ± 0.10 | 1.08 ± 0.48 |
| Total length apical dendrite | 3531 ± 236 | 3128 ± 138 | 2606 ± 47 | 2008 ± 177 | 1776 ± 258 | 3161 ± 115 | 3471 ± 511 | 6530 ± 310** | 1757 ± 190 | 3853 ± 371 | 1083 ± 144 | 2470 ± 533 |
| Total length basal dendrite | 1649 ± 153 | 2791 ± 234 | 2609 ± 330 | 3123 ± 248 | 2596 ± 339 | 5213 ± 221*/** | 3355 ± 274 | 4580 ± 220*/** | 3074 ± 177 | 1685 ± 250 | 1597 ± 131 | 2464 ± 187 |
| Ratio length | 2.43 ± 0.39** | 1.16 ± 0.09 | 1.07 ± 0.19 | 0.69 ± 0.13 | 0.72 ± 0.15 | 0.64 ± 0.04 | 1.07 ± 0.14 | 1.49 ± 0.10 | 0.57 ± 0.04 | 2.52 ± 0.48** | 0.72 ± 0.14 | 1.07 ± 0.27 |
| Sum length | 5180 ± 265 | 5919 ± 308 | 5215 ± 315 | 5130 ± 154 | 4372 ± 324 | 8373 ± 220** | 6826 ± 667 | 11110 ± 368** | 4830 ± 358 | 5538 ± 469 | 2680 ± 73 | 4934 ± 437 |
| Soma size (μm2) | 204 ± 7 | 188 ± 9 | 178 ± 14 | 193 ± 20 | 294 ± 18** | 292 ± 11** | 311 ± 18*/** | 361 ± 19** | 188 ± 18 | 186 ± 20 | 185 ± 9 | 218 ± 24 |
| Number of apical braches | 29 ± 2**,b | 19 ± 2 | 16 ± 1 | 11 ± 1 | 10 ± 2 | 17 ± 1 | 20 ± 3 | 38 ± 2** | 10 ± 3 | 25 ± 3 | 14 ± 2 | 15 ± 5 |
| Number of basal branches | 20 ± 2 | 26 ± 1 | 24 ± 3 | 24 ± 3 | 22 ± 2 | 31 ± 1 | 23 ± 2 | 37 ± 2** | 14 ± 1 | 20 ± 3 | 18 ± 3 | 21 ± 4 |
| Total number of branches | 49 ± 3 | 45 ± 1 | 39 ± 5 | 35 ± 2 | 31 ± 1 | 48 ± 1 | 43 ± 4 | 75 ± 2** | 24 ± 3 | 45 ± 4 | 32 ± 2 | 36 ± 4 |
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Note: aL2 is different from all other subtypes with the exception of L5 RS neurons (P = 0.09).
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bL2 is different from all other subtypes with the exception of L6 tall neurons (P = 0.23).
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For clarity, the results of the post hoc comparison of groups is only given (values in bold followed with asterisks) when a cell type is different from all other cell types, unless otherwise specified. *P < 0.05, **P < 0.01.