TABLE 1.Differences in the firing properties of GIN and YFP-16 neurons are preserved in dissociated cells
|
|
|||
| YFP-16 | GIN | P | |
|
|
|||
| AP width, ms | 0.69 ± 0.19 | 0.88 ± 0.13 | <0.0001 |
| AP height, mV | 73.2 ± 7.9 | 70.3 ± 7.8 | 0.11 |
| AP threshold, mV | –50.5 ± 7.9 | –49.1 ± 3.8 | 0.07 |
| AP peak, mV | 37.8 ± 6.2 | 36.2 ± 5.4 | 0.24 |
| AP Rise rate, V/s | 263 ± 66 | 218 ± 63 | 0.005 |
| AP Fall rate, V/s | 142 ± 39 | 107 ± 33 | 0.0001 |
| ADP, V/s | 0.96 ± 0.8 | 0.47 ± 0.7 | 0.008 |
| AHP, mV | 33.3 ± 3.0 | 35.4 ± 4.7 | 0.04 |
| Spont FR, Hz | 13.9 ± 5.8 | 10.5 ± 5.1 | 0.02 |
| CV | 0.077 ± 0.099 | 0.063 ± 0.05 | 0.88 |
| Rinput, MΩ | 1626 ± 760 | 1685 ± 582 | 0.48 |
| Capacitance, pF | 8.0 ± 2.3 | 7.2 ± 1.6 | 0.13 |
| Rseries, MΩ | 8.1 ± 3.3 | 9.8 ± 3.9 | 0.07 |
Averages and SD of parameters used to quantify action potential (AP) and firing properties in dissociated YFP-16 (n = 39) and GIN (n = 35) neurons. Spont FR, spontaneous firing rate: CV, coefficient of variation of the inter-spike interval. Cell health,
size, and quality of the recording, assessed with input resistance (Rinput), whole cell capacitance, and series resistance (Rseries), respectively, were not different between the two populations of neurons.