Locations of thalamic injections and postsynaptic cortical cells for the TC studies
| Mouse | Center of thalamic injection | Loci of cells from which TC responses were measured | |||
|---|---|---|---|---|---|
| AP position (posterior from bregma, mm) | Thalamic nucleus | AP position (anterior from bregma, mm) | Cortical area | Postsynaptic cell class | |
| 1 | -1.82 | VM | 1.94 | Cg1/M2 | LS L1 (n = 1), other L1 (n = 1) |
| 2 | -1.22 | VM/Sub | 1.98 | PrL | Other L1 (n = 1), Pyr L2/L3 (n = 1) |
| 3 | -1.06 | AM | 1.98 | Cg1 | LS L1 (n = 1), other L1 (n = 1) |
| 4 | -0.82 | AM | 0.38 | Cg1 | LS L1 (n = 1), other L1 (n = 1) |
| 5 | -0.70 | AM | 2.22 | Cg1 | Other L1 (n = 1), Pyr L2/L3 (n = 1) |
| 1.78 | PrL | Other L1 (n = 1) | |||
| 0.98 | Cg2 | Pyr L2/L3 (n = 2) | |||
| 6 | -1.06 | AM | 1.18 | Cg2 | LS L1 (n = 1), other L1 (n = 1) |
| 0.74 | Cg2 | Other L1 (n = 2) | |||
| 7 | -1.06 | AM/Sub | 1.70 | PrL | Pyr L2/L3 (n = 1) |
| 1.34 | Cg1 | Other L1 (n = 1), Pyr L2/L3 (n = 1) | |||
| 8 | -0.82 | AM/Re | 1.98 | PrL | NLS L1 (n = 1), Pyr L2/L3 (n = 1) |
| 1.70 | PrL | LS L1 (n = 1), NLS L1 (n = 1), Pyr L2/L3 (n = 1) | |||
| 9 | -1.06 | AM/CM | 1.94 | PrL | LS L1 (n = 1), Pyr L2/L3 (n = 1) |
| 1.18 | Cg1 | Other L1 (n = 2) | |||
| 10 | -1.06 | AM/PC/CM | 1.98 | Cg1 | LS L1 (n = 1), NLS L1 (n = 1) |
| 1.54 | Cg1 | LS L1 (n = 1) | |||
| 0.98 | Cg2 | NLS L1 (n = 1), other L1 (n = 1) | |||
| 11 | -1.06 | AM/IAM/CM | 1.18 | Cg2 | Pyr L2/L3 (n = 2) |
| 0.74 | Cg2 | LS L1 (n = 1), other L1 (n = 1) | |||
| 0.14 | Cg2 | NLS L1 (n = 1) | |||
| 12 | -1.06 | AM/IAM | 0.38 | Cg2 | LS L1 (n = 1) |
| 13 | -1.06 | PC/AM | -0.10 | Cg1 | LS L1 (n = 1), other L1 (n = 1) |
| Cg2 | Other L1 (n = 2) | ||||
| 14 | -0.70 | Re | 1.98 | Cg1 | Other L1 (n = 2) |
| 1.54 | PrL | Other L1 (n = 1), Pyr L2/3 (n = 1) | |||
| 15 | -1.46 | Rh | 1.18 | Cg2 | Other L1 (n = 2) |
| 16 | -1.34 | Rh | 1.98 | Cg1 | NLS L1 (n = 1), other L1 (n = 1) |
| 1.18 | Cg2 | Other L1 (n = 1) | |||
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Locations of thalamic virus injections for each mouse (columns 2 and 3) were determined from EYFP expression in slices (>8 d after injections), aided by the mouse brain atlas of Paxinos and Franklin (2001). Centers of the injections are indicated by both anteroposterior (AP) position relative to bregma and the thalamic nuclei involved. The injections included in the study were centered on ventromedial, anteromedial, reuniens, or rhomboid thalamic nuclei and resulted in concentrated terminal arbors in outer L1 of mPFC. Injections involving mediodorsal thalamic nucleus also resulted in outer L1 projections, but the mediodorsal thalamic nucleus projections had additional terminations in L3 (n = 2; data not shown). Given the potential for light scattering to L3 axons during high-intensity L1 stimulation, we chose not to study TC synapses in these animals. Locations of the cortical cells from which TC synaptic responses were measured are shown in columns 4 and 5, and cell types are shown in column 6. For each mouse/injection, the recordings were generally targeted to the region of most intense terminal labeling within L1 of mPFC. AM, Anteromedial thalamic nucleus; Cg1 and Cg2, cingulate cortices, areas 1 and 2; CM, central medial thalamic nucleus; IAM, interanteromedial thalamic nucleus; LS L1, late-spiking interneuron in L1; MD, mediodorsal thalamic nucleus; M2, secondary motor cortex; NLS L1, non-late-spiking interneuron in L1; Other L1, uncategorized interneuron in L1; PC, paracentral thalamic nucleus; PrL, prelimbic cortex; Pyr L2/L3, pyramidal cell in L2 or L3; Re, reuniens thalamic nucleus; Rh, rhomboid thalamic nucleus; Sub, submedius thalamic nucleus; VM, ventromedial thalamic nucleus (Paxinos and Franklin, 2001).