Gene | Sequence | Product length (bp) |
---|---|---|
TPH2 | Forward: 5′-TACGGCACCGAGCTTGAC-3′ | 84 |
Reverse: 5′-TGGCCACATCCACCAAATAC-3′ | ||
GAD67 | Forward: 5′-CAAGTTCTGGCTGATGTGGA-3′ | 231 |
Reverse: 5′-GCCACCCTGTGTAGCTTTTC-3′ | ||
NSE | Forward: 5′-ATAGTGGGCGATGACCTGAC-3′ | 200 |
Reverse: 5′-ATGAACGTGTCCTCCGTTTC-3′ | ||
VIAAT | Forward: 5′-GGGCTGGAACGTGACAAA-3′ | 65 |
Reverse: 5′-GGAGGATGGCGTAGGGTAG-3′ |
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Primer sequences and estimated product lengths for RT-PCR are shown. The primers are used for both the first and second PCR amplifications. The concentration of the primers is 1 μm for the first PCR and 10 μm for the second PCR.