AboutNeuron TypesElectrophysiology PropertiesArticlesFAQsData/APIGet Involved

Article: Distinct neurochemical and functional properties of GAD67-containing 5-HT neurons in the rat dorsal raphe nucleus.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Shikanai H; Yoshida T; Konno K; Yamasaki M; Izumi T; Ohmura Y; Watanabe M; Yoshioka M
J. Neurosci., 2012


Table 1.

The primer sequences and estimated product lengths for single-cell RT-PCR

Gene Sequence Product length (bp)
TPH2 Forward: 5′-TACGGCACCGAGCTTGAC-3′ 84
Reverse: 5′-TGGCCACATCCACCAAATAC-3′
GAD67 Forward: 5′-CAAGTTCTGGCTGATGTGGA-3′ 231
Reverse: 5′-GCCACCCTGTGTAGCTTTTC-3′
NSE Forward: 5′-ATAGTGGGCGATGACCTGAC-3′ 200
Reverse: 5′-ATGAACGTGTCCTCCGTTTC-3′
VIAAT Forward: 5′-GGGCTGGAACGTGACAAA-3′ 65
Reverse: 5′-GGAGGATGGCGTAGGGTAG-3′
  • Primer sequences and estimated product lengths for RT-PCR are shown. The primers are used for both the first and second PCR amplifications. The concentration of the primers is 1 μm for the first PCR and 10 μm for the second PCR.


Report miscurated data

Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source