Article: COX-2-Derived Prostaglandin E2 Produced by Pyramidal Neurons Contributes to Neurovascular Coupling in the Rodent Cerebral Cortex.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Lacroix A; Toussay X; Anenberg E; Lecrux C; Ferreirós N; Karagiannis A; Plaisier F; Chausson P; Jarlier F; Burgess SA; Hillman EM; Tegeder I; Murphy TH; Hamel E; Cauli B
J. Neurosci., 2015

Table 2.

PCR primers

Genes accession no.	First PCR primers	Size, bp	Second PCR nested primers	Size, bp
GFAP	Sense, 454: `AAGCTCCAAGATGAAACCAACCT`	631	Sense, 509 `ACAGACAGGAGGCGGATGAA`	263
NM_017009	(Lambolez et al., 1992)		(Lecrux et al., 2011)
	Antisense, 1066: `CGATCTCGATGTCCAGGGC`		Antisense, 749 `GGACCGATACCACTCTTCTGTTT`
	(Lambolez et al., 1992)		(Lecrux et al., 2011)
S100β	Sense, 45: `CCATCAGTATTCAGGGAGAGAGGG`	166	Sense, 58: `GGGAGAGAGGGTGACAAGCA`	132
NM_013191.1	(Lecrux et al., 2011)		(Lecrux et al., 2011)
	Antisense, 191: `GTCACACTCCCCATCCCCAT`		Antisense, 170: `TTCGTCCAGCGTCTCCATCA`
	(Lecrux et al., 2011)		(Lecrux et al., 2011)
vGluT1	Sense, 361: `GGCTCCTTTTTCTGGGGGTAC`	259	Sense, 373: `TGGGGGTACATTGTCACTCAGA`	201
NM_053859.1	(Gallopin et al., 2006)		(Gallopin et al., 2006)
	Antisense, 600: `CCAGCCGACTCCGTTCTAAG`		Antisense, 553:ATGGCAAGCAGGGTATGTGAC
	(Gallopin et al., 2006)		(Gallopin et al., 2006)
GAD65	Sense, 713: `TCTTTTCTCCTGGTGGTGCC`	391	Sense, 743: `TGTACGCCATGCTCATTGCC`	312
NM_012563.1	(Bochet et al., 1994)		(Gallopin et al., 2006)
	Antisense, 1085: `CCCCAAGCAGCATCCACAT`		Antisense, 1032: `CAGCTACAGCCAAGAGAGGATCA`
	(Bochet et al., 1994)		(Gallopin et al., 2006)
GAD67	Sense, 529: `TACGGGGTTCGCACAGGTC`	599	Sense, 581: `TGGATATCATTGGTTTAGCTGGC`	508
NM_017007.1	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 1109: `CCCCAAGCAGCATCCACAT`		Antisense, 1065: `TCACATATGTCTGCAATCTCCTGG`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
NOS-1	Sense, 2816: `AACTGGGAGGGGAGAGGATTC`	548	Sense, 2829: `GAGGATTCTGAAGATGAGGGAGG`	517
NM_052799.1	(Cauli et al., 2004)		Antisense, 3323: `CCTTCCAATTACTGATGACACCC`
	Antisense, 3323: `GGGTGGGAGGCGAGATTCAT`
	(Cauli et al., 2004)
CB	Sense, 134: `AGGCACGAAAGAAGGCTGGAT`	432	Sense, 166: `CCTGAGATGAAAACCTTTGTGG`	249
NM_031984.2	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 544: `TCCCACACATTTTGATTCCCTG`		Antisense, 392: `CACGGTCTTGTTTGCTTTCTCTA`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
PV	Sense, 115: `AAGAGTGCGGATGATGTGAAGA`	388	Sense, 151: `CTGGACAAAGACAAAAGTGGCT`	244
NM_022499.2	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 480: `TTGTTTCTCCAGCATTTTCCAG`		Antisense,376: `AGAAGGGCTGAGATGGGGC`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
CR	Sense, 142: `CTGGAGAAGGCAAGGAAAGGT`	309	Sense, 157: `AAAGGTTCTGGCATGATGTCC`	265
NM_053988.1	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 429: `AGGTTCATCATAGGGACGGTTG`		Antisense, 401: `TCAGGAGGTCGGACAGAAATC`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
NPY	Sense, -45: `GCCCAGAGCAGAGCACCC`	359	Sense, -21: `CAGAGACCACAGCCCGCC`	302
NM_012614.1	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 292: `CAAGTTTCATTTCCCATCACCA`		Antisense, 261: `CTTCAAGCCTTGTTCTGGGG`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
VIP	Sense, 189: `TGCCTTAGCGGAGAATGACA`	287	Sense, 208: `ACGCCCTATTATGATGTGTCCAG`	214
NM_053991.1	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 456: `CCTCACTGCTCCTCTTCCCA`		Antisense, 402: `TTTGCTTTCTAAGGCGGGTG`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
SOM	Sense, 43: `ATCGTCCTGGCTTTGGGC`	208	Sense, 75: `GCCCTCGGACCCCAGACT`	150
NM_012659.1	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 231: `GCCTCATCTCGTCCTGCTCA`		Antisense, 207: `TGGGGCAAATCCTCAGGC`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
CCK	Sense, 177: `CGCACTGCTAGCCCGATACA`	216	Sense, 192: `ATACATCCAGCAGGTCCGCA`	151
NM_012829.1	(Cauli et al., 1997)		(Gallopin et al., 2006)
	Antisense, 373: `TTTCTCATTCCGCCTCCTCC`		Antisense, 320: `TGGGTATTCGTAGTCCTCAGCAC`
	(Cauli et al., 1997)		(Gallopin et al., 2006)
COX-1	Sense, 27: `GTTTCCCCTGCTGCTGCTC`	359	Sense, 137: `AGGGTGTCTGTGTCCGCTTC`	185
NM_017043.1	(Lecrux et al., 2011)		(Lecrux et al., 2011)
	Antisense, 366: `GGCTGGGGATAAGGTTGGAC`		Antisense, 301: `GGCATTCACAAACTCCCAGAT`
	(Lecrux et al., 2011)		(Lecrux et al., 2011)
COX-2	Sense, 199: `CTGAAGCCCACCCCAAACAC`	247	Sense, 202: `AAGCCCACCCCAAACACAGT`	206
NM_017232.2	(Lecrux et al., 2011)		(Lecrux et al., 2011)
	Antisense, 426: `TGGGAGTTGGGCAGTCATCA`		Antisense, 384: `CTGGTGTAGTAGGAGAGGTTGGAG`
	(Lecrux et al., 2011)		(Lecrux et al., 2011)
mPGES1	Sense, 9: `CCTGGGTTTGGTGATGGAGAA`	362	Sense, 36: `GGTGCTCCCCGCCTTTCT`	290
NM_021583.2	Antisense, 350: `GGTTCATTTTGCCCAGGTAGG`		Antisense, 303: `TGAGGACCACGAGGAAATGTATC`
mPGES2	Sense, 358: `GACTTCCACTCCCTGCCCT`	348	Sense, 381: `GGTGGTAGAGGTGAATCCCG`	291
NM_001107832.1	Antisense, 686: `CTGCCGCCACTTCATCTCCT`		Antisense, 652: `TTTCCGCCATACATCTGCTG`
cPGES	Sense, -18: `GCCCTCTGCCCCGTTCAC`	305	Sense, 26: `ACGACCGAAGGGACTATGTATT`	187
NM_001130989.1	Antisense, 266: `GCTTTGCCCTTTCCTTTGTTA`		Antisense, 191: `CTGTCCGTTCTTTTATGCTTGG`
PGIS	Sense, 1142: `GTGACCGCCTTCTCCTCTTTC`	240	Sense, 1174: `CCCCAGAAGGACCCAGAAATC`	190
NM_031557.2	Antisense, 1361: `TCAGCAGCAGGACCACAAACT`		Antisense, 1342: `ACTGTTTGATGCTGTTGATGGC`

Note: Position 1, first base of the start codon.

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Inferred neuron-electrophysiology data values

Neuron Type	Neuron Description	Ephys Prop	Extracted Value	Standardized Value	Content Source