Article: Local-Circuit Phenotypes of Layer 5 Neurons in Motor-Frontal Cortex of YFP-H Mice.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Yu J; Anderson CT; Kiritani T; Sheets PL; Wokosin DL; Wood L; Shepherd GM Front Neural Circuits, 2008
resting Vm (mV) | input resistance (Mohm) | capacitance (pF) | ISI ratio (1st / last) | Adaptation index | |
---|---|---|---|---|---|
YFP+ | −62 ± 5 | 153 ± 90 | 127 ± 80 | 0.49 | 1.071239 |
YFP− | −62 ± 4 | 145 ± 63 | 139 ± 64 | 0.33 | 0.713871 |
: We did not find significant differences between YPF-positive (n = 24) and YFP-negative (n = 19) neurons in basic electrophysiological parameters, including resting membrane potential (YFP+, −62 ± 5 mV; YFP−, −62 ± 4 mV; mean ± s.d.; p > 0.05, t-test), input resistance (YFP+, 153 ± 90 MΩ; YFP−, 145 ± 63 MΩ; mean ± s.d.; p > 0.05, t-test), or capacitance (YFP+, 127 ± 80 mV; YFP−, 139 ± 64 mV; mean ± s.d.; p > 0.05, t-test). Comparison of spike frequency adaptation in YFP-positive and YFP-negative neurons. (A) Spike latencies were used to calculate instantaneous firing rates for the neurons in each group, and these were binned in 100-ms bins for averaging. The plot shows the mean (±s.e.m.) instantaneous firing rate as a function of time after step onset. Inset shows representative examples of spike patterns of YFP-positive (gray trace) and YFP-negative (black trace) neurons evoked by depolarizing current steps. Scale bars, 0.2 s, 50 mV. (B) Average adaptation index for YFP-negative (open) and YFP-positive (gray) neurons. The group averages were significantly different (*, p |