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Article: Hyperexcitability of cultured spinal motoneurons from presymptomatic ALS mice.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Kuo JJ; Schonewille M; Siddique T; Schults AN; Fu R; Bär PR; Anelli R; Heckman CJ; Kroese AB
J. Neurophysiol., 2004



TABLE 1.Motoneuron properties
Neonate in Cultured Slices
Embryonic in Primary Culture

Control
G93A
Control
G93A
n 23 16 27 40
Cell properties
   Soma diameter, µm 32 ± 6 32 ± 4 27 ± 2 27 ± 3
   Resting membrane.potential, mV -52 ± 11 -57 ± 11 -70 ± 5 -69 ± 6
   Input resistance, MΩ 52 ± 18 41 ± 24 360 ± 189 397 ± 237
Spike characteristics
   Duration, ms 2.5 ± 1 2.4 ± 0.9 2.0 ± 0.5 1.9 ± 0.6
   Amplitude, mV 77 ± 14 71 ± 15 77 ± 7 72 ± 10*
   Threshold, mV -45 ± 10 -48 ± 5 -49 ± 5 -48 ± 5
f-I relation
   Slope, sp · s-1 · pA-1 0.25 ± 0.12 0.42 ± 0.14*** 0.11 ± 0.05 0.16 ± 0.09**
   fmax, sp · s-1 95 ± 25 125 ± 29** NM NM
   I0, pA 770 ± 640 580 ± 290 34 ± 44 26 ± 36
Post-spike AHP
   Amplitude, mV 10 ± 2 10 ± 2 21 ± 5 20 ± 6
Post-train AHP n = 11 n = 11
   Amplitude, mV/spike 0.6 ± 0.3 0.6 ± 0.3 NM NM
   Time constant, ms
87 ± 19
84 ± 24
NM
NM
Comparison of properties of G93A and control motoneurons. Values are means ± SD. Neonatal (NC) neurons were held at -70 mV by DC current clamp to prevent generation of action potentials by the spontaneous excitatory synaptic activity. Differences between mean values were determined by t-test. Significance is indicated by *(P < 0.05), **(P < 0.005), and ***(P < 0.0005). The difference between the embryonic (EC) G93A and control spike amplitude is unlikely to affect the excitability, as there was no correlation (r2 = 0.0001) between excitability (f-I slope) and spike amplitude. NM, not measured; AHP, afterhyperpolarization.

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Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Spinal cord intermediate horn motor neuron sympathetic cell diameter 32.0 ± 6.0 (23) 32.0 (um) Data Table
Spinal cord intermediate horn motor neuron sympathetic resting membrane potential (mV) -52.0 ± 11.0 (23) -52.0 (mV) Data Table
Spinal cord intermediate horn motor neuron sympathetic input resistance (MΩ) 52.0 ± 18.0 (23) 52.0 (MΩ) Data Table
Spinal cord intermediate horn motor neuron sympathetic spike width (ms) 2.5 ± 1.0 (23) 2.5 (ms) Data Table
Spinal cord intermediate horn motor neuron sympathetic spike amplitude (mV) 77.0 ± 14.0 (23) 77.0 (mV) Data Table
Spinal cord intermediate horn motor neuron sympathetic spike threshold (mV) -45.0 ± 10.0 (23) -45.0 (mV) Data Table
Spinal cord intermediate horn motor neuron sympathetic FI slope 0.25 ± 0.12 (23) 0.25 (Hz/nA) Data Table
Spinal cord intermediate horn motor neuron sympathetic rheobase (pA) 770.0 ± 640.0 (23) 770.0 (pA) Data Table
Spinal cord intermediate horn motor neuron sympathetic AHP amplitude (mV) 10.0 ± 2.0 (23) 10.0 (mV) Data Table
Spinal cord intermediate horn motor neuron sympathetic AHP amplitude 0.6 ± 0.3 (23) 0.6 (mV) Data Table