Size Category | Large | Medium | Small | ||||||
---|---|---|---|---|---|---|---|---|---|
Treatment | Sham | DRGL4 | DRGL5 | Sham | DRGL4 | DRGL5 | Sham | DRGL4 | DRGL5 |
Cell size, um | 58.7 ± 1.7 | 58.9 ± 1.3 | 57.9 ± 0.9 | 38.1 ± 0.7 | 37.6 ± 0.7 | 38.4 ± 0.6 | 25.5 ± 0.6 | 25.3 ± 0.6 | 26.7 ± 0.6 |
n | 44 | 44 | 96 | 40 | 34 | 47 | 72 | 74 | 39 |
RMP, mV | −60.1 ± 1.0 | −60.3 ± 1.1 | −57.1 ± 0.6 | −56.6 ± 1.3 | −54.1 ± 1.2 | −53.5 ± 1.0 | −52.3 ± 0.9 | −51.4 ± 0.9 | −50.3 ± 1.2 |
n | 44 | 44 | 96* † | 40 | 34 | 47 | 72 | 74 | 39 |
Rin, Mohm | 20.2 ± 1.7 | 27.4 ± 3.0 | 31.7 ± 2.1 | 29.9 ± 2.7 | 27.5 ± 2.9 | 38.5 ± 3.7 | 39.7 ± 2.8 | 44.1 ± 3.9 | 50.2 ± 4.6 |
n | 36 | 29* | 711-160 | 33 | 31 | 41† | 66 | 63 | 36 |
Curr. thre., nA | 1.55 ± 0.22 | 1.16 ± 0.16 | 0.72 ± 0.05 | 0.88 ± 0.12 | 0.84 ± 0.09 | 0.52 ± 0.04 | 0.62 ± 0.05 | 0.64 ± 0.06 | 0.62 ± 0.06 |
n | 31 | 21* | 771-160 † | 31 | 31 | 441-160 † | 58 | 53 | 37 |
Vol. thre., mV | −39.9 ± 1.3 | −45.9 ± 1.6 | −45.9 ± 0.9 | −32.4 ± 2.0 | −33.8 ± 3.0 | −40.5 ± 2.0 | −21.4 ± 1.2 | −23.6 ± 1.8 | −34.4 ± 2.2 |
n | 33 | 241-160 | 551-160 | 26 | 22 | 31* † | 50 | 41 | 211-160 1-164 |
APD50, ms | 0.49 ± 0.06 | 0.67 ± 0.10 | 0.99 ± 0.06 | 0.96 ± 0.08 | 1.56 ± 0.22 | 1.46 ± 0.12 | 2.73 ± 0.27 | 2.78 ± 0.18 | 2.80 ± 0.25 |
n | 23 | 23* | 581-160 † | 25 | 22* | 27* | 32 | 40 | 21 |
dV/dt r., mV/ms | 296 ± 27.3 | 248 ± 28.4 | 199 ± 10.3 | 202 ± 17.3 | 138 ± 12.2 | 145 ± 9.0 | 109 ± 7.5 | 96.7 ± 7.3 | 99.1 ± 10.1 |
n | 23 | 23 | 581-160 | 26 | 22* | 27* | 32 | 40 | 21 |
dV/dt f., mV/ms | −181 ± 18.6 | −133 ± 11.4 | −88.5 ± 5.6 | −112 ± 12.0 | −74.3 ± 7.8 | −63.2 ± 4.1 | −60.9 ± 4.4 | −48.1 ± 4.0 | −41.1 ± 2.6 |
n | 23 | 231-160 | 581-160 † | 26 | 22* | 27* | 32 | 40* | 211-160 |
AHP50, ms | 3.23 ± 0.35 | 4.11 ± 0.68 | 3.82 ± 0.36 | 4.21 ± 0.72 | 5.54 ± 0.83 | 5.03 ± 0.53 | 8.71 ± 1.12 | 7.22 ± 0.55 | 6.31 ± 0.54 |
n | 23 | 23 | 50 | 23 | 21 | 25 | 31 | 41 | 18 |
AHP amp., mV | −9.9 ± 0.6 | −9.7 ± 0.5 | −7.0 ± 0.4 | −10.8 ± 1.0 | −12.1 ± 1.1 | −8.78 ± 0.8 | −10.5 ± 0.9 | −11.1 ± 0.7 | −11.9 ± 0.8 |
n | 23 | 23 | 511-160 1-164 | 23 | 21 | 25 | .31 | 41 | 18 |
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Values are means ± SE, and n = sample size; SNL: L5 spinal nerve ligation and transection; DRG L4 and L5: intact (L4) and axotomized (L5) ganglia from SNL rats, Sham: ganglion from Sham-operated rats; Cell size: mean diameter of neuron; RMP: resting membrane potential; Curr. thre.: current threshold; Vol. thre.: voltage threshold; APD50: action potential (AP) duration at half width, dV/dt r.: maximum rising rate of AP; dV/dt f.: maximum falling rate of AP; AHP50: afterhyperpolarization duration at half width; AHP amp.: amplitude of afterhyperpolarization.
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↵* P < 0.05,
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↵F1-160 P < 0.01, DRG L4 or DRG L5 vs. Sham;
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↵† P < 0.05,
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↵F1-164 P < 0.01, DRG L5 vs. DRG L4.