Table 3.
Comparison of carbachol-induced changes in RMP and shoulder-potential width in hippocampal CA3 pyramidal neurons expressing different mAChR subtypes
| Genotype | Layer | n | ΔRMP, mV | ΔShoulder Width, ms |
|---|---|---|---|---|
| Wild-type (WT) | CA3 | 12 | +1.9 ± 2.0 | +1,600 ± 221 |
| M3/M5 DKO | CA3 | 11 | −0.1 ± 1.5 | +998 ± 194 |
| M1 KO | CA3 | 11 | +0.6 ± 1.2 | +313 ± 88* |
| M1/M3 DKO | CA3 | 7 | −2.9 ± 1.0* | +2 ± 2* |
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Values are means ± SE. Cholinergic responses evoked by bath application of carbachol (10 μM for 5 min) in CA3 pyramidal neurons. Boldface numbers indicate P < 0.05 for carbachol-induced effects within individual cell types (repeated-measures ANOVA with post-tests: baseline, carbachol, and wash). Asterisks indicate P < 0.01 when compared with values in wild-type neurons (one-way ANOVA with post-tests).