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Article: Antisense suppression of potassium channel expression demonstrates its role in maturation of the action potential.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Vincent A; Lautermilch NJ; Spitzer NC J. Neurosci., 2000
Properties of control and antisense-treated neurons
| Parameters | Controls | AS to xKv3.1 | Significance of comparison | |
|---|---|---|---|---|
| NF | FAS | FAS* | ||
| Membrane potential (mV) | −45.3 ± 0.6 (12) | −44.4 ± 1.0 (9) | −45.4 ± 0.6 (6) | NS |
| Capacitance (pF/μm2) | 22.9 ± 1.2 (12) | 22.5 ± 1.7 (9) | 21.4 ± 2.0 (6) | NS |
| Input resistance (GΩ) | 1.2 ± 0.1 (12) | 1.4 ± 0.2 (9) | 1.1 ± 0.1 (6) | NS |
| I Kv density (pA/μm2, +40 mV) | 1.2 ± 0.2 (12) | 1.1 ± 0.2 (9) | 0.5 ± 0.1 (6) | FAS* vs FAS p < 0.05 |
| FAS* vs controlp < 0.01 | ||||
| I Kv t 1/2 max (msec, +40 mV) | 1.0 ± 0.1 (12) | 1.0 ± 0.1 (9) | 1.6 ± 0.1 (6) | FAS* vs FAS p < 0.05 |
| FAS* vs control p < 0.01 | ||||
| I Ca (peak) (pA/μm2) | 0.3 ± 0.1 (6) | 0.3 ± 0.1 (7) | NS | |
| AP duration (at 0 mV) (msec) | 1.9 ± 0.1 (9) | 1.9 ± 0.2 (9) | 3.5 ± 0.6 (6) | FAS* vs FAS p < 0.01 |
| FAS* vs control p < 0.01 | ||||
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Properties of the resting membrane, whole-cell currents, and action potentials recorded from nonfluorescent neurons (NF, control) and from fluorescent neurons (F, receiving AS) at 1 d in culture. Fluorescent cells were assigned to the AS* category when mean values were different by >2 sd from values for NF controls in the same culture dish. The rest were placed in the AS category. Values are mean ± SEM for (n) neurons from ≥3 experiments. NS, Not significantly different.
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
|---|---|---|---|---|---|
| Other | Xenopus spinal neurons | input resistance (GΩ) | 1.2 ± 0.1 (12) | 1200.0 (MΩ) | Data Table |
| Other | Xenopus spinal neurons | resting membrane potential (mV) | -45.3 ± 0.6 (12) | -45.3 (mV) | Data Table |
| Other | Xenopus spinal neurons | spike width | 1.9 ± 0.1 (9) | 1.9 (ms) | Data Table |
| Other | Xenopus spinal neurons | cell capacitance | 22.9 ± 1.2 (12) | 22.9 (pF) | Data Table |