Table 1
Solutions used to calibrate BTC with NPE under high ionic strength
| Concentrations (mm) | |||||
|---|---|---|---|---|---|
|
|
|||||
| A | B | C | D | E | |
| K-Asparate* [1.23] | 300 | 300 | 305 | 310 | 360 |
| KCl | 65 | 65 | 65 | 65 | 10 |
| K-Hepes (pH 7.3) | 150 | 150 | 150 | 150 | 150 |
| Glucose | 11 | 11 | 11 | 11 | 11 |
| K2-Glutathione | 10 | 10 | 10 | 10 | 10 |
| CaCl2 | 0 | 7.5 | 25 | 35 | 45 |
| K3-DPTA* [4.5] | 0 | 50 | 50 | 50 | 0 |
| K2-EGTA* [∼7] | 50 | 0 | 0 | 0 | 0 |
| BTC* | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| NPE* [∼7] (CaCl2) | 5(0) | 5(5) | 5(5) | 5(5) | 5(5) |
| Measured [Ca2+] (μm) | |||||
|---|---|---|---|---|---|
|
|
|||||
| A | B | C | D | E | |
| 111 % solution | < 0.01 | 5.88 | 33.1 | 74.6 | 6440 |
| Pre-flash | < 0.01 | 5.95 | 33.1 | 74.5 | 6320 |
| Post-flash | < 0.01 | 7.52 | 37.9 | 87.4 | 6610 |
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Top part of table shows composition of calibration solutions A–E.
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↵* Ca2+-binding compound with pK values shown in square brackets. Bottom part of table shows measured [Ca2+] in the 111 % solution containing the first eight constituents and used to make final solutions, or calculated using pK values in final solutions before (Pre-flash) and after (Post-flash) single full strength (no neutral density filter) photolysis of NPE.