Table 1.
P2X3, P2X2, or both P2X3 and P2X2 mRNA expressed colon senory neurons
| A. DNA primers used for PCR amplification | |||
|---|---|---|---|
| Target Gene/Predicted Size | Outer Primers | Nested Primers | Genbank No. |
| P2X3 | GCTCCGTAGAAGAAGATGGAGA | TGTCCTAAGAGGATCCTGTACC | NM_145526 |
| 251 bp, 141 bp | CTGTGTGACCATGTTAGGGATG | GGCATCTAGCACATAGAAGTGG | |
| P2X2 | CTCTTCAGTAACCATGTCCACG | GAAGATAGGCATCTTGCTCTGG | NM_153400 |
| 233 bp, 113bp | CCGGAAGACAGCTCTAATTTGG | GGGATCCTATGAGGAGTTCTGT | |
| B. Colon sensory neurons expressing P2X3, P2X2 or both P2X3 and P2X2 mRNA | ||||
|---|---|---|---|---|
| P2X3 Only, % | Both P2X3 and P2X2, % | P2X2 Only, % | Neither P2X3 or P2X2, % | |
| Saline | ||||
| LS | 33/59; 55.9 | 20/59; 33.9 | 3/59; 5.1 | 3/59; 5.1 |
| TL | 31/54; 57.4 | 20/54; 37.0 | 3/54; 5.6 | 3/54; 5.6 |
| Zymosan | ||||
| LS | 25/58; 43.1 | 29/58; 50.0 | 2/58; 3.4 | 2/58; 3.4 |
| TL | 33/55; 60.0 | 18/55; 32.7 | 1/55; 1.8 | 3/55; 5.5 |
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Sequence of P2X3, and P2X2 mRNA are indicated with 5′ at let. a, adenosine; c, cytosine; g, guanosine; t, thymidine; Outer primers were used for the first round of PCR; nested primers were used for the second round. Sequences used for primer design are indicated at right. bp, base pairs.
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Colon sensory neurons expressing P2X3, P2X2 or both P2X3 and P2X2 mRNA are shown. The ratios of mRNA expression are calculated per total number of GAPDH mRNA-positive neurons in each group. LS, lumbosacral; TL, thoracolumbar.