Properties of mEPSCs in dentate granule cells after pilocarpine-induced status epilepticus
| CGC | GC-SE | HEGC | |
|---|---|---|---|
| Frequency, Hz | 0.16 ± 0.02 | 0.22 ± 0.03 | 0.38 ± 0.08*† |
| Interevent interval, s | 6.0 ± 0.4 | 4.6 ± 0.3 | 2.0 ± 0.1‡§ |
| Amplitude, pA | 8.48 ± 0.18 | 9.86 ± 0.24‡ | 10.38 ± 0.35‡ |
| 10–90% rise time, ms | 1.89 ± 0.07 | 2.19 ± 0.08 | 2.52 ± 0.08‡ |
| τ, ms | 2.22 ± 0.09 | 3.51 ± 0.17‡ | 4.15 ± 0.16‡ |
| Charge transfer, fC | 22.83 ± 0.93 | 38.73 ± 2.23‡ | 57.74 ± 3.42‡ |
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Values are means ± SE. mEPSC frequency was determined for 9 (CGC) or 10 (GC-SE and HEGC) cells per group. Other parameters were determined for 268 (CGC), 397 (GC-SE), or 785 (HEGC) events recorded from the indicated number of cells. mEPSCs were recorded in the presence of 30 μM bicuculline and 1 μM TTX for 2.5 min at 22–24°C. Recordings were made with a CsCl-based internal solution at a holding potential of −70 mV.
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↵* Significantly different from CGC at P < 0.05 (Newman-Keuls test after 1-way ANOVA yielded P < 0.025).
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↵† Significantly different from GC-SE at P < 0.05 (Newman-Keuls test).
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↵‡ Significantly different from CGC at P < 0.001 (Kolmogorov-Smirnov test).
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↵§ Significantly different from GC-SE at P < 0.001 (Kolmogorov-Smirnov test). mEPSC, miniature excitatory posysynaptic current; CGC, normotopic granule cells from control rats; GC-SE, normotopic granule cells from rats subjected to pilocarpine-induced status epilepticus; HEGC, hilar ectopic granule cells.