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Article: A blueprint for the spatiotemporal origins of mouse hippocampal interneuron diversity.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Tricoire L; Pelkey KA; Erkkila BE; Jeffries BW; Yuan X; McBain CJ J. Neurosci., 2011
Table 1.
List of primary antibodies
| Antigen | Antigen details | Host | Clonality | Dilution | Supplier | Catalog no. | Purification method | Reference/specificity |
|---|---|---|---|---|---|---|---|---|
| CCK | Gastrin-17 | Mouse | Monoclonal | 1:1000 | Dr. G. Ohning (CURE, University of California, Los Angeles, Los Angeles, CA) | 9303 | Ascites fluids | Recognizes gastrin and CCK, but gastrin is absent of telencephalon (Ohning et al., 1996) |
| CR | Recombinant rat calretinin | Rabbit | Polyclonal | 1:1000 | Millipore | AB5054 | Antiserum | Western blot shows a single band at 32 kDa molecular weight (manufacturer). Labeling pattern as published in previous reports (Fonseca et al., 1995) |
| CoupTFII | Recombinant human COUP-TF II (amino acids 43–64) | Mouse | Monoclonal | 1:200 | Perseus Proteomics; clone H7147 | PP-H7147-00 | Ascites fluids | Tested by Western blot and KO animal (Qin et al., 2007) |
| GFP | Recombinant GFP | Chicken | Polyclonal | 1:2000 | Aves Labs | GFP-1020 | IgY fraction | No immunohistochemistry signal can be detected from the GFP-negative Cre+;Z/EG−/− mouse tissue |
| PV | Purified frog muscle parvalbumin | Mouse | Monoclonal | 1:1000 | Sigma-Aldrich; clone PARV-19 | P3088 | Ascites fluids | Labeling pattern as with rabbit antiserum (Swant; PV-28) (Kubota et al., 2011) |
| PV | Rat muscle parvalbumin | Rabbit | Polyclonal | 1:1000 | Swant | PV-28 | Antiserum | No labeling in KO mouse (Schwaller et al., 1999) |
| M2R | Amino acids 225–359 | Rat | Monoclonal | 1:500 | Millipore; clone M2-2-B3 | MAB367 | Protein A purified | Levey et al., 1995 |
| nNOS | Recombinant human neuronal nitric oxide synthase | Rabbit | Polyclonal | 1:1000 | Millipore | AB5380 | Peptide affinity chromatography | Labeling pattern as published with other antibodies (Fuentealba et al., 2008) |
| Reelin | Recombinant reelin amino acids 164–496 | Mouse | Monoclonal | 1:1000 | Millipore; clone G10 | MAB5364 | Protein A purified | Recognizes the H epitope near the N terminus of the protein (de Bergeyck et al., 1998). No staining in the reeler mouse (de Bergeyck et al., 1997) |
| SOM | Synthetic cyclic (1–14) somatostatin conjugated to bovine thyroglobulin | Rat | Monoclonal | 1:200 | Millipore; clone YC7 | MAB354 | Culture supernatant | Labeling pattern as published with other antibodies (Eyre et al., 2009) |
| SOM | Same as the rat antibody | Rabbit | Polyclonal | 1:500 | Dako | A0566 | Antiserum | Labeling pattern as published with the rat antibody (our observation) |
| VIP | Porcine VIP | Guinea pig | Polyclonal | 1:1000 | Peninsula Laboratories | T-5030 | Antiserum | Labeling pattern as published with other antibodies (Eyre et al., 2009) |
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
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