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Article: A blueprint for the spatiotemporal origins of mouse hippocampal interneuron diversity.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Tricoire L; Pelkey KA; Erkkila BE; Jeffries BW; Yuan X; McBain CJ
J. Neurosci., 2011


Table 2.

Sequences of PCR primers

Marker GenBank no. First PCR primers Size Second PCR primers Size
5HT3AR NM_013561.2 Sense, 889: TCAGACACACTGCCAGCAAC 485 Sense, 981: GACCATCTTCATTGTGCGGC 337
(Htr3a) Antisense, 1356: TATCCCACCCGCAGCCAG Antisense, 1300: GAAGTGGCGGATGGAGGA
CCK NM_031161.3 Sense, 16: TGTCTGTGCGTGGTGATGGC 554 Sense, 192: ATACATCCAGCAGGTCCGCAA 237
Antisense, 546: GCATAGCAACATTAGGTCTGGGAG Antisense, 408: CAGACATTAGAGGCGAGGGGT
CNP NM_009923.2 Sense, −52: CCCCGGAGACATAGTACCCGCAA 437 Sense, −22: GACGGCGGTGCGCCCACT 318
Antisense, 364: GCTCCCGCTCGTGGTTGGTAT Antisense, 275: GCCTCGGAGAAGTCTGCCCGA
CoupTFII NM_009697.3 Sense, 605: AGCCTAACAACATCATGGGC 404 Sense, 686: ACATCCCCTTCTTCCCTGAC 318
(Nr2f2) Antisense, 989: GCTTTCCACATGGGCTACAT Antisense, 984: CCACATGGGCTACATCAGAC
CR NM_007586.1 Sense, 63: TTGATGCTGACGGAAATGGGTA 265 Sense, 141: GCTGGAGAAGGCAAGGAAGG 151
(Calb2) Antisense, 327: CAAGCCTCCATAAACTCAGCG Antisense, 271: ATTCTCTTCGGTCGGCAGGAT
Enk NM_001002927.2 Sense, 92: CTAAATGCAGCTACCGCCTG 407 Sense, 119: CAGGCGACATCAATTTCCTG 202
(Penk) Antisense, 479: GTTGTCTCCCGTTCCCAGTA Antisense, 301: TACCGTTTCATGAAGCCTCC
GAD65 NM_008078.2 Sense, 99: CCAAAAGTTCACGGGCGG 375 Sense, 219: CACCTGCGACCAAAAACCCT 248
(Gad2) Antisense, 454: TCCTCCAGATTTTGCGGTTG Antisense, 447: GATTTTGCGGTTGGTCTGCC
GAD67 NM_008077.4 Sense, 83: ATGATACTTGGTGTGGCGTAGC 253 Sense, 159: CAATAGCCTGGAAGAGAAGAGTCG 177
(Gad1) Antisense, 314: GTTTGCTCCTCCCCGTTCTTAG Antisense, 314: GTTTGCTCCTCCCCGTTCTTAG
GFAP NM_010277.3 Sense, 523: AAGCCACCCTGGCTCGTGTG 407 Same as first PCR 304
Antisense, 910: CTGTTCGCGCATTTGCCGCT Antisense, 807: TTGGCTTGGCGGAGCAGCTC
Lhx6 NM_008500.2 Sense, 150: TGCCATGGCTCAGTCAGACGCA 473 Sense, 199: GACGAAGGTAGAGCCTCCCCATGT 206
Antisense, 599: CCTTCTCCTCAACGAGGCCGAATT Antisense, 383: TGCCTCAGCGATGTGCGACACA
MBPa NM_010777.3 Sense, 399: ATGGCATCACAGAAGAGACC 354, 422, 442 Same as first PCR
Antisense, 721: CATGGGAGATCCAGAGCGGC 479, 574 Same as first PCR
NG2 NM_139001.2 Sense, 4841: CAGCACAGGCGCTGACCCTC 408 Sense, 4873: ACCGGGTGGTAAGAGGCCCC 340
(Cspg4) Antisense, 5229: TGCCGACTGCGCTGAGATGC Antisense, 5193: AGGTTGGCGGCATCAAGGGC
nNOS NM_008712.2 Sense, 1668: CCTGGGGCTCAAATGGTATG 373 Sense, 1742: CCTGTCCCTTTAGTGGCTGGTA 236
(Nos1) Antisense, 2021: CACAATCCACACCCAGTCGG Antisense, 1957: GATGAAGGACTCGGTGGCAGA
Npas1 NM_008718.2 Sense, 945: CATCCTTGCTTGTGAGAGCA 436 Sense, 1024: TTTGTTCATGGACAGGATGC 227
Antisense, 1361: AGGGTCCTTGTCCTTGTCCT Antisense, 1231: TCCAGGGGTGTTTGACTACC
Npas3 NM_013780.2 Sense, 730: GCCAGTTCAGCATCTTCCTC 472 Sense, 937: ATCATGGGTCTGGTGGTTGT 204
Antisense, 1155: TCAGCAAGTCCAGGTGACTG Antisense, 1121: TACATCTTCGGCATGGATGA
NPY NM_023456.2 Sense, 16: CGAATGGGGCTGTGTGGA 297 Sense, 38: CCCTCGCTCTATCTCTGCTCGT 220
Antisense, 289: AAGTTTCATTTCCCATCACCACAT Antisense, 236: GCGTTTTCTGTGCTTTCCTTCA
PV NM_013645.3 Sense, 104: GCCTGAAGAAAAAGAACCCG 275 Sense, 122: CGGATGAGGTGAAGAAGGTGT 163
(Pvalb) Antisense, 275: AATCTTGCCGTCCCCATCCT Antisense, 265: TCCCCATCCTTGTCTCCAGC
S100b NM_009115.3 Sense, 48: CAGTACTCCGGGCGAGAGGGT 405 Sense, 112: ACAACGAGCTCTCTCACTTCCTGG 248
Antisense, 431: AGGGGGTTGGGGTTTCATCCCC Antisense, 336: GCTGTGCCTCCTCTTGTGACCCTC
SOM NM_009215.1 Sense, 1: ATGCTGTCCTGCCGTCTCCA 250 Sense, 41: GCATCGTCCTGGCTTTGGG 170
(Sst) Antisense, 231: GCCTCATCTCGTCCTGCTCA Antisense, 191: GGGCTCCAGGGCATCATTCT
VGluT1 NM_182993.2 Sense, 597: CCCTTAGAACGGAGTCGGCT 593 Sense, 621: ACGACAGCCTTTTGCGGTTC 367
(Slc17a7) Antisense, 1169: TATCCGACCACCAGCAGCAG Antisense, 968: CAAAGTAGGCGGGCTGAGAG
VGluT3b NM_182959.3 Sense, 42: AGGAGTGAAGAATGCCGTGGGAGAC 574 Sense, 94: GATGGGACCAATGAAGAGGAAGAT 384
(Slc17a8) Antisense, 594: ACCCTCCACCAGACCCTGCAAA Antisense, 453: TGAAATGAAGCCACCGGGAATTTGT
VIP NM_011702.2 Sense, 219: TTATGATGTGTCAAGAAATGCCAG 424 Sense, 253: GGAGTTTTCACCAGCGATTACAG 225
Antisense, 618: TTTTATTTGGTTTTGCTATGGAAG Antisense, 455: CTCACTGCTCCTCTTTCCATTCA

  • Note: Position 1, first base of the start codon. The name of the gene is listed in parentheses when it differs from the usual name of the marker.

  • ↵aFrom Ye et al. (2003).

  • ↵bFrom Dal Bo et al. (2004).


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Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source