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Article: Different fear states engage distinct networks within the intercalated cell clusters of the amygdala.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Busti D; Geracitano R; Whittle N; Dalezios Y; Mańko M; Kaufmann W; Sätzler K; Singewald N; Capogna M; Ferraguti F J. Neurosci., 2011
List of primary antibodies used and specification of dilution, source, and specificity
| Antibodies | Antigen | Species | Dilution | Reactions | Source | Characterization of the primary antibody | References |
|---|---|---|---|---|---|---|---|
| μ opioid receptor | Synthetic peptide corresponding to amino acids 384–398 predicted from cloned rat MOR1 | Rabbit | 1:20,000 | a, g | ImmunoStar; catalog #24216; lot #607102 | Preadsorption with MOR peptide (384–398) at 10 μg/ml completely eliminates labeling. The specificity of the antiserum was determined by immunolabeling of transfected cells, Western blot analysis, and immunoisolation studies. | Likhtik et al., 2008 |
| Tyrosine hydroxylase | Tyrosine hydroxylase | Rabbit | 1:3000 | b | Chemicon; catalog #AB152; lot #23060094 | Immunohistochemistry: stains both fresh-frozen and paraffin-embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Western blot; immunoprecipitation. It does not work on paraffin-embedded, human tissue. | Lode et al., 2000; Prohaska and Brokate, 2001 |
| α3 GABAA receptor subunit | Amino acidic sequence 1–11 in the N- terminal domain | Rabbit | 1:1500 | c | Kindly provided by Prof. Sieghart (Medical University of Vienna, Vienna, Austria) | Tested immunohistochemically on fixed brain tissue of both wild-type and α3 GABAA knock-out mice (kindly provided by Dr. J. M. Fritschy). | Pirker et al., 2003 |
| mGlu1α receptor | Amino acidic sequence 945–1127 | Guinea pig | 1:500 | f, g, A | Kindly provided by Dr. Watanabe (Hokkaido University, Sapporo, Japan) | The antibody was tested in mouse by immunohistochemistry on fixed brain tissue. | Tanaka and al., 2000; Ferraguti et al., 2004 |
| 1:1000 | m | ||||||
| FoxP2 | Synthetic peptide conjugated to KLH derived from within residues 700 to the C terminus of Himan FOXP2 | Rabbit | 1:2000 | d | Abcam; catalog #ab16046 | Nuclear localization. This antibody gave positive signal in HEK293 whole-cell lysate. It reacts with mouse and human. Not tested in other species. | Campbell et al., 2009; Enard et al., 2009; Regad et al., 2009 |
| GABA | GABA–BSA | Rabbit | 1:7000 | e | Sigma-Aldrich; catalog #A2052 | It shows positive binding with GABA and GABA–KLH in a dot blot assay and negative binding with BSA. | Menegola et al., 2008 |
| Neurokinin 1 receptor | Synthetic peptide amino acid sequence (385–407) of the C terminus of the rat | Rabbit | 1:3000 | h | Chemicon; catalog #AB5060; lot #23030368 and #0512016863 | Immuhistochemistry: preabsorbtion of the antiserum with the immunogen peptide eliminates all immunostainings. | Piggins et al., 2001; Mátyás et al., 2004 |
| Parvalbumin | Rat muscle parvalbumin | Rabbit | 1:1000 | i | Swant; catalog #PV-28; lot #5.5 | Western blotting (no cross-reaction was observed with any other known Ca2+ binding proteins). Immunobinding assay: the title of the serum was in range of 1:5000–1:10,000 when 1 ng of antigen was applied. | Kägi et al., 1987 |
| Parvalbumin | Carp muscle parvalbumin | Mouse | 1:1000 | l | Sigma; catalog #P-3171; lot #017H4821 | The immunospecificity for parvalbumin was determined by indirect immunoperoxidase staining and immunoblotting. Specific reaction with parvalbumin of culture nerve cells and tissue originating from human, monkey, rat, mouse, chicken, and fish. | McDonald and Betette, 2001; Tanahira et al., 2009 |
| Calbindin | Rat calbindin D-28k | Rabbit | 1:10,000 | m | Swant; catalog #CB-38a; lot #9.03 | In immunoblots it recognizes a single band of ∼ 28 kDa. Antiserum CB38 immunolabels a subpopulation of neurons in the normal brain with high efficiency but does not stain the brain of calbindin D-28 knock-out mice. | Airaksinen et al., 1997 |
| anti-GFP | Raised against GFP isolated from Aequorea victoria | Rabbit | 1:1000 | n | Invitrogen; catalog #A11122 | IgG fraction purified by ion-exchange chromatography. It is useful for immunoprecipitation, immunohistochemistry, and Western blotting. | Ausubel et al., 1992; Conn, 1999 |
| Egr-1/Zif268 | Raised against a peptide mapping at the C terminus of Egr-1 of human origin | Rabbit | 1:5000 | o | Santa Cruz Biotechnology; catalog #sc-189 | It is useful for detection of Egr-1 p82 of mouse, rat, and human origin by Western blotting, immunoprecipitation, immunofluorescence, and solid-phase ELISA. | Hefner et al., 2008; Whittle et al., 2009 |
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Reactions performed for light microscopy are indicated by small letters, whereas reactions performed for electron microscopy are indicated by capital letters. MOR, μ opioid receptor; KLH, keyhole limpet hemocyanin; a, b, c, d, e, o, Light microscopy, biotinylated anti-rabbit; f, light microscopy, anti-guinea pig Alexa Fluor 488 and Cy3–streptavidin; g, light microscopy, anti-rabbit Cy3, and anti-guinea pig Alexa Fluor 488; h, i, light microscopy, anti-rabbit Alexa Fluor 488 and Cy3–streptavidin; l, light microscopy, anti-mouse Alexa Fluor 488 and Cy3–streptavidin; m, n, light microscopy, anti-rabbit Alexa Fluor 488 and Cy3-streptavidin.
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
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