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Article: Calcium imaging in single neurons from brain slices using bioluminescent reporters.

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Drobac E; Tricoire L; Chaffotte AF; Guiot E; Lambolez B
J. Neurosci. Res., 2010

Table I. Kinetics of Bioluminescent Sensors Responses to Ca2+ 1 mM In Vitro*
[Mg2+] (mM)GAGOGAN26D
τ Rise (msec)τ Decay (sec)τ Rise (msec)τ Decay (sec)τ Rise (msec)τ Decay (sec)
  • *

    Onset time constants were obtained by fitting the 50–90% rise phase, except for GO, where fits were done on the 70–99% rise phase. Decay time constants were determined by fitting the 90–10% decay phase, except for GA and GO in the presence of Mg2+ 2 mM, where fits were performed on the 90–20% decay phase.


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