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Article: Altered neuron excitability and synaptic plasticity in the cerebellar granular layer of juvenile prion protein knock-out mice with impaired motor control.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Prestori F; Rossi P; Bearzatto B; Lainé J; Necchi D; Diwakar S; Schiffmann SN; Axelrad H; D'Angelo E J. Neurosci., 2008
Excitable properties
| C57BL/6J (n = 22) | C57 × Sv129 (n = 23) | PrP0/0 fast (n = 12) | PrP0/0 slow (n = 8) | |
|---|---|---|---|---|
| Vrest (mV) | −60.7 ± 2.8* | −60.5 ± 2.5* | −52.3 ± 8.3 | −47.0 ± 5.0 |
| APOS (mV) | 53.6 ± 2.6** | 50.0 ± 2.4** | 49.1 ± 4.0*** | 29.0 ± 2.6 |
| APAHP (mV) | 19.8 ± 1.8*** | 16.5 ± 1.2*** | 18.4 ± 1.2*** | 10.3 ± 2.0 |
| APthr (mV) | −42.7 ± 2.0*** | −48.3 ± 1.2** | −44.9 ± 2.1*** | −34.7 ± 1.3 |
| (−36.0 ± 2.2*) | (−36.6 ± 0.7***) | (−36.1 ± 1.8*) | (−29.1 ± 1.8) | |
| APHW (ms) | 1.2 ± 0.15*** | 0.99 ± 0.08*** | 1.4 ± 0.2*** | 4.0 ± 0.7 |
| EPSPampl (mV) | 15.6 ± 1.5 | 14.9 ± 1.3 | 16.9 ± 1.9 | 15.8 ± 3.1 |
| EPSPτ (ms) | 26.0 ± 2.7*** | 30.5 ± 3.6* | 33.3 ± 5.2* | 49.9 ± 9.3 |
| Iin(peak) (pA/pF) | −309.8 ± 27.7** | −307.5 ± 28.3** | −232.9 ± 37.4*** | −83.3 ± 12.3 |
| Iin(I/V peak) (mV) | −26.5 ± 2.3*** | −27.9 ± 1.9* | −27.5 ± 1.5* | −12.5 ± 3.6 |
| Iout-t(+20) (pA/pF) | 265.6 ± 25.2*** | 244.5 ± 14.3** | 238.2 ± 23.4*** | 141.1 ± 11.2 |
| Iout-p(+20) (pA/pF) | 183.4 ± 22.7*** | 170.8 ± 13.9*** | 198.2 ± 36.9* | 107.5 ± 10.8 |
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The recorded granule cells had a slightly different resting membrane potential (Vrest), which was then adjusted with constant current injection (−70 mV for EPSPs; −80 mV for action potentials) to normalize current-clamp recordings. The action potential overshoot (APOS), duration at half-width (APHW), and afterhyperpolarization (APAHP) are calculated from threshold (APthr). The reported APthr corresponds to spike prepotential (the values in parentheses are the upstroke threshold). In the same cells, EPSP amplitude (EPSPampl) and decay time constant (EPSPτ) are indicated. The table also reports values for the maximum transient inward current (Iin(peak)) along with the corresponding membrane potential (Iin(I/V peak)), the transient outward current at +20 mV (Iout-t(+20)), and the persistent outward current at +20 mV (Iout-p(+20)). The number of observations is indicated and statistical significance is reported in comparisons with slow-spiking PrP0/0 granule cells.
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↵*p < 0.05, **p < 0.001, ***p < 0.01, unpaired t test.
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
|---|---|---|---|---|---|
| Cerebellum granule cell | resting membrane potential (mV) | -60.7 ± 2.8 (22) | -60.7 (mV) | Data Table | |
| Cerebellum granule cell | spike amplitude (mV) | 53.6 ± 2.6 (22) | 53.6 (mV) | Data Table | |
| Cerebellum granule cell | AHP amplitude (mV) | 19.8 ± 1.8 (22) | 19.8 (mV) | Data Table | |
| Cerebellum granule cell | spike threshold (mV) | -42.7 ± 2.0 (22) | -42.7 (mV) | Data Table | |
| Cerebellum granule cell | spike half-width (ms) | 1.2 ± 0.15 (22) | 1.2 (ms) | Data Table |