Article: Fibroblast growth factor homologous factors control neuronal excitability through modulation of voltage-gated sodium channels.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Goldfarb M; Schoorlemmer J; Williams A; Diwakar S; Wang Q; Huang X; Giza J; Tchetchik D; Kelley K; Vega A; Matthews G; Rossi P; Ornitz DM; D'Angelo E Neuron, 2007
Table 1Summary of Electrophysiological Data
∗Significant deficit (p < 0.05) by ANOVA. ∗∗Significant deficit (p < 0.01) by ANOVA. #Significant versus Fhf4−/− (p < 0.05) by t test.
A. Cerebellar Slice Recordings—Physiological Solutions | ||||
Wild-Type | Fhf1−/− | Fhf4−/− | Fhf1−/−Fhf4−/− | |
# Cells recorded | 7 | 4 | 4 | 7 |
Rin (GΩ) | 1.53 ± 0.70 | 1.40 ± 0.42 | 0.47 ± 0.20 | 0.95 ± 0.51 |
Cm (pF) | 4.62 ± 0.96 | 4.24 ± 1.40 | 4.47 ± 1.35 | 4.35 ± 1.16 |
Rs (MΩ) | 26.6 ± 14.0 | 25.2 ± 7.2 | 19.1 ± 6.3 | 22.3 ± 8.9 |
Iin-peak at −10 mV (pA) | −1012 ± 287 | −933 ± 360 | −1860 ± 707 | −1033 ± 359 |
Iout-peak at +20 mV (pA) | 999 ± 421 | 844 ± 446 | 956 ± 440 | 974 ± 950 |
Vrest (mV) | −69.4 ± 8.4 | −72.3 ± 4.9 | −55.3 ± 10.7 | −59.9 ± 6.3 |
Vthreshold (mV) | −49.6 ± 4.9 | −50.9 ± 9.8 | −43.4 ± 4.4 | −40.2 ± 8.7∗ |
Ithreshold x Rin (mV) | 29.6 ± 6.5 | 27.4 ± 7.2 | 30.4 ± 4.6 | 39.4 ± 11.4 |
Vahp (mV) | −59.8 ± 7.3 | −61.9 ± 4.5 | −50.4 ± 3.9 | −46.8 ± 2.5∗ |
Maximum # spikes in 800 ms | 47.0 ± 20.9 | 48.0 ± 20.6 | 8.5 ± 4.7∗∗ | 2.3 ± 2.2∗∗# |
B. Cultured Granule Cell Recordings—Sodium Currents Only | ||||
Wild-Type (# Cells) | Fhf1−/−Fhf4−/− (# Cells) | t Test | ||
Cm (pF) | 4.74 ± 0.95 (9) | 3.86 ± 0.83 (11) | ||
Rs (MΩ) | 12.1 ± 3.5 (9) | 13.5 ± 7.5 (11) | ||
Frequency voltage clamp (kHz) | 2.99 ± 0.58 (9) | 3.58 ± 0.95 (11) | ||
Transient INa-Peak at −15 mV (pA) | −431 ± 166 (9) | −536 ± 326 (11) | ||
Resurgent INa 5 mV to −85 mV (pA) | −117 ± 78 (9) | −134 ± 76 (9) | ||
Persistent INa at −35 mV (pA) | −26 ± 7 (9) | −12 ± 6 (9) | p < 0.0003 | |
V1/2 Activation (mV) | −33.3 ± 4.2 (9) | −36.5 ± 7.5 (11) | ||
k Activation (mV) | 5.2 ± 0.7 (9) | 4.6 ± 1.2 (11) | ||
V1/2 Inactivation (mV) | −59.1 ± 4.9 (8) | −72.8 ± 4.3 (9) | p < 0.00003 | |
k Inactivation (mV) | −5.8 ± 0.6 (8) | −5.3 ± 0.6 (9) | ||
τFast Inactivation at −15 mV (msec) | 1.10 ± 0.24 (6) | 0.49 ± 0.08 (10) | p < 0.001 | |
τFast Inactivation at −5 mV (msec) | 0.64 ± 0.12 (6) | 0.33 ± 0.05 (10) | p < 0.0003 | |
τRecovery at −85 mV (ms) | 3.80 ± 0.80 (7) | 5.88 ± 0.88 (7) | p < 0.001 | |
C. Cerebellar Slice Recordings—Sodium Currents Only | ||||
Wild-Type (# Cells) | Fhf1−/−Fhf4−/− (# Cells) | t Test | ||
Cm (pF) | 4.58 ± 0.99 (14) | 3.89 ± 0.67 (15) | ||
Rs (MΩ) | 16.0 ± 6.7 (14) | 17.9 ± 10.4 (15) | ||
Frequency voltage clamp (kHz) | 2.61 ± 1.24 (14) | 2.88 ± 1.03 (15) | ||
Transient INa-Peak at −15 mV (pA) | −907 ± 367 (11) | −551 ± 201 (10) | p < 0.02 | |
Resurgent INa 5 mV to −85 mV (pA) | −65 ± 46 (9) | −28 ± 26 (10) | ||
V1/2 Inactivation (mV) | −55.6 ± 2.0 (6) | −69.9 ± 5.3 (8) | p < 0.00005 | |
k Inactivation (mV) | −4.8 ± 0.9 (6) | −5.2 ± 0.9 (8) | ||
τ Recovery at −85 mV (ms) | 3.04 ± 0.20 (4) | 7.22 ± 3.31 (6) | p < 0.025 |
Inferred neuron-electrophysiology data values
Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
---|---|---|---|---|---|
Cerebellum granule cell | input resistance (GΩ) | 1.53 ± 0.7 (7) | 1530.0 (MΩ) | Data Table | |
Cerebellum granule cell | cell capacitance (pF) | 4.62 ± 0.96 (7) | 4.62 (pF) | Data Table | |
Cerebellum granule cell | access resistance (MΩ) | 26.6 ± 14.0 (7) | 26.6 (MΩ) | Data Table | |
Cerebellum granule cell | resting membrane potential (mV) | -69.4 ± 8.4 (7) | -69.4 (mV) | Data Table | |
Cerebellum granule cell | spike threshold (mV) | -49.6 ± 4.9 (7) | -49.6 (mV) | Data Table | |
Cerebellum granule cell | AHP voltage (mV) | -59.8 ± 7.3 (7) | -59.8 (mV) | Data Table |