CGC | GC-SE | HEGC | |||||||
---|---|---|---|---|---|---|---|---|---|
Before | + Ligand | Before | + Ligand | Before | + Ligand | ||||
THDOC | |||||||||
Frequency, Hz | 3.1 ± 0.6 | 3.8 ± 0.4 | 3.1 ± 0.7 | 3.0 ± 0.9 | 1.1 ± 0.3 | 1.6 ± 0.7 | |||
Amplitude, pA | 30.3 ± 4.0 | 29.1 ± 2.5 | 27.6 ± 3.5 | 23.4 ± 4.2 | 22.8 ± 4.9 | 25.0 ± 3.1 | |||
10–90% rise time, ms | 1.7 ± 0.3 | 2.0 ± 0.2 | 2.4 ± 0.2 | 2.4 ± 0.3 | 2.8 ± 0.9 | 2.3 ± 0.4 | |||
τ, ms | 10.6 ± 0.9 | 13.3 ± 3.2† | 10.2 ± 0.8 | 9.6 ± 1.6 | 10.8 ± 2.8 | 10.9 ± 3.1 | |||
Charge transfer, fC | 301 ± 52 | 354 ± 44 | 265 ± 39 | 221 ± 58 | 293 ± 88 | 214 ± 40 | |||
L-655708 | |||||||||
Frequency, Hz | 2.5 ± 0.3 | 2.2 ± 0.4 | 2.4 ± 0.9 | 2.2 ± 0.8 | 3.0 ± 0.6 | 2.3 ± 0.5 | |||
Amplitude, pA | 28.4 ± 3.8 | 25.7 ± 3.1 | 27.0 ± 3.6 | 21.2 ± 5.6 | 37.5 ± 8.6 | 25.7 ± 6.6 | |||
10–90% rise time, ms | 2.8 ± 0.9 | 2.3 ± 0.4 | 1.9 ± 0.2 | 2.1 ± 0.1 | 1.8 ± 0.2 | 2.0 ± 0.3 | |||
τ, ms | 9.5 ± 0.3 | 7.8 ± 0.4‡ | 10.9 ± 0.6 | 9.0 ± 0.9 | 12.3 ± 1.5 | 8.0 ± 1.3‡ | |||
Charge transfer, fC | 234 ± 29 | 175 ± 20‡ | 293 ± 46 | 192 ± 56* | 426 ± 95 | 191 ± 52‡ |
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Values are means ± SE for 5 (CGC/THDOC), 6 (CGC/L-655,708), 6 (GC-SE/THDOC), 7 (GC-SE/L-655,708), 6 (HEGC/THDOC), or 7 (HEGC/L-655,708) cells per group. The number of sIPSCs recorded during a 2.5-min period and the properties of averaged sIPSCs were determined before and during exposure to the test compound at 22–24°C. Where indicated, the ligand altered the sIPSC property significantly at
-
↵* P < 0.05.
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↵† P < 0.02, or
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↵‡ P < 0.01 (paired t-test). sIPSC, spontaneous inhibitory postsynaptic current. See Table 1 for other abbreviations.