Article: Effects of variations in hippocampal slice preparation protocol on the electrophysiological stability, epileptogenicity and graded hypoxia responses of CA1 neurons.
Table 1Effect of incubation protocol on the stability of evoked fields in vibratome-cut slices
28–30°C
19–21°
Cold-shock
Orthodromic PS (% control)
(n=7)
(n=8)
(n=8)
30 min
100.2±6.5
101.4±4.9
113.4±9.8a
60 min
102.9±7.8
101.3±5.4
119.3±13.2b
Antidromic PS (% control)
(n=7)
(n=7)
(n=8)
30 min
100.6±2.6
99.2±6.9
101.7±9.4
60 min
98.9±3.2
96.7±10.9
99.0±11.6
Orthodromic field (# PS's)
(n=7)
(n=8)
(n=8)
0 min
1.0±0.0
2.1±0.6
3.3±0.9
60 min
1.0±0.0
2.1±0.6
4.1±0.6a
Antidromic field (# PS's)
(n=7)
(n=7)
(n=8)
0 min
1.0±0.0
1.4±0.5
1.4±0.7
60 min
1.0±0.0
1.4±0.5
1.8±1.0
Errors represent standard deviation. aSignificant difference with respect to the data collected at 0 min. bSignificant difference with respect to the data collected at 0 min and 30 min.
