Article: Effects of variations in hippocampal slice preparation protocol on the electrophysiological stability, epileptogenicity and graded hypoxia responses of CA1 neurons.
Table 2Summary of the whole-cell properties of neurons in CS or room temperature-incubated, vibratome-cut slices
19–21°C incubation (n=5)
Cold-shock (n=6)
Resting mp (mV)
−60.88±3.24
−63.08±4.21
RN (MΩ)
43.12±7.34
40.56±6.55
ap threshold (mV)
−47.54±2.22
−46.25±1.61
ap height (mV)
80.91±6.08
79.18±3.41
ap duration (ms)
1.51±0.12
1.56±0.18
IPSP (mV)
6.05±3.09
1.77±1.08a
# synaptically evoked ap's
1.40±0.55
3.50±0.55a
The errors represent standard deviation. aSignificant difference with respect to data obtained from slices incubated at 19–21°C. The mp's at which the IPSP's were evoked were not significantly different between the room temperature-incubated (−59.56±5.07 mV) and CS neurons (−59.63±2.85 mV).
