TABLE 2.Effect of K+ channel blockers on spontaneous activity
|Axotomy + ACSF||Axotomy + TEA||Axotomy + 4-AP|
|Number of animals||3||3||3|
|Incidence of Spontaneous activity, %||21.1 ± 0.5||31.5 ± 1.0||31.8 ± 2.9|
|P value vs. ACSF||—||0.0001||0.0001|
|Total number of fibers recorded||503||555||576|
Incidence of spontaneous activity determined on post-operative day (POD) 7 after perfusion of the injured nerve with K+ blockers TEA (10 mM) or 4-aminopyridine (4-AP; 10 mM), or with vehicle (ACSF). The pump was still in place at the time spontaneous activity was measured. SEs are based on comparison between different animals. P values are based on Fisher's exact tests on the combined data. There was no significant difference between the TEA and 4-AP groups.