Table 2.
Activation and inactivation of Na+ currents in nucleated patches of neurons and whole cell recordings of tsA cells
| Current-density (pA/pF) | Steady-state activation | Steady-state inactivation | Trec at -100 mV | |||||
|---|---|---|---|---|---|---|---|---|
| V1/2 (mV) | kV | n | V1/2 (mV) | kV | n | |||
| Nucleated patches of inhibitory neurons in primary cortical neurons | ||||||||
| WT | -684.6 ± 93.2 | -29.6 ± 0.5 | -6.7 ± 0.1 | 22 | -63.1 ± 0.8 | 5.2 ± 0.2 | 22 | 4.1 ± 0.4 |
| Scn1aRH/+ | -757.4 ± 142.1 | -29.4 ± 0.8 | -6.5 ± 0.2 | 20 | -63.6 ± 1.1 | 5.6 ± 0.4 | 20 | 4.4 ± 0.5 |
| Nucleated patches of inhibitory CA1 neurons in acute slices | ||||||||
| WT | -143.3 ± 29.5 | -33.0 ± 1.4 | -6.8 ± 0.2 | 10 | -68 ± 1.1 | 9.1 ± 0.4 | 9 | 3.9 ± 1.0 |
| Scn1aRH/+ | -116.9 ± 12.0 | -33.3 ± 1.1 | -6.4 ± 0.4 | 15 | -66.6 ± 1.4 | 9.6 ± 0.4 | 15 | 5.1 ± 1.3 |
| Scn1aRH/RH | -106.7 ± 17.5 | -34.4 ± 1.1 | -6.6 ± 0.4 | 9 | -71.0 ± 1.1 | 11.8 ± 1.5 | 8 | 6.6 ± 1.6 |
| Whole cell patch-clamp recordings of transfected tsA cells | ||||||||
| WT | -421.8 ± 54.6 | -35.1 ± 2.2 | 6.2 ± 0.4 | 9 | -73.0 ± 1.3 | 4.6 ± 0.1 | 8 | 5.3 ± 0.6 |
| R1648H | -251.2 ± 53.5* | -34.4 ± 1.7 | 7.1 ± 0.3 | 11 | -73.6 ± 0.8 | 7.1 ± 0.5* | 11 | 1.8 ± 0.2* |
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Data are presented as means ± SEM. n, Number of recorded neurons. TRec, time constant of recovery from fast inactivation.
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↵*p < 0.001, WT vs R1648H. Mann-Whitney rank-sum test for cases with two groups compared (WT vs Scn1aRH/+ or R1648H mutation) and ANOVA on ranks with Dunn's post hoc test for comparing WT, Scn1aRH/+, and Scn1aRH/RH mice.