Peak δF/F | T0.5 (s) | Frequency (min-1) | No. of mice | |
---|---|---|---|---|
Basic properties | ||||
Global waves | ||||
Control | 1.81 ± 0.08 (n = 26 waves, 189 locations) | 4.94 ± 0.16 (n = 26 waves, 189 locations) | 0.87 ± 0.22 (n = 6 cells) | 4 |
+TTX | 1.66 ± 0.07 (n = 34 waves, 244 locations) | 4.47 ± 0.13 (n = 34 waves, 244 locations) | 1.13 ± 0.31 (n = 6 cells) | 4 |
p | 0.07 | 0.02 | 0.136 | |
Local waves | ||||
Control | 1.72 ± 0.09 (n = 34 waves, 103 locations) | 4.22 ± 0.19 (n = 34 waves, 103 locations) | 1.13 ± 0.30 (n = 6 cells) | 4 |
+TTX | 1.64 ± 0.08 (n = 39 waves, 106 locations) | 4.14 ± 0.22 (n = 39 waves, 106 locations) | 1.17 ± 0.08 (n = 6 cells) | 4 |
p | 0.558 | 0.5 | 0.58 | |
Microdomains | ||||
Control | 1.29 ± 0.07 (n = 145 events) | 3.01 ± 0.16 (n = 145 events) | 4.83 ± 0.99 (n = 6 cells) | 4 |
+TTX | 1.20 ± 0.05 (n = 151 events) | 2.98 ± 0.11 (n = 151 events) | 5.03 ± 1.04 (n = 6 cells) | 4 |
p | 0.40 | 0.16 | 0.713 | |
Store depletion | ||||
Global waves | ||||
Control | 1.77 ± 0.07 (n = 23 waves, 161 locations) | 5.77 ± 0.37 (n = 23 waves, 161 locations) | 0.77 ± 0.18 (n = 6 cells) | 3 |
+CPA | - (n = 0 waves) | - (n = 0 waves) | 0 ± 0 | 3 |
p | ND | ND | 0.0084 | |
Local waves | ||||
Control | 1.60 ± 0.06 (n = 50 waves, 103 locations) | 5.15 ± 0.34 (n = 50 waves, 103 locations) | 1.67 ± 0.22 (n = 6 cells) | 3 |
+CPA | 1.60 ± 0.09 (n = 3 waves, 4 locations) | 6.78 ± 1.14 (n = 3 waves, 4 locations) | 0.1 ± 0.07 (n = 6 cells) | 3 |
p | 0.98 | 0.10 | 0.0011 | |
Microdomains | ||||
Control | 1.14 ± 0.03 (n = 189 events) | 4.0 ± 0.21 (n = 189 events) | 6.3 ± 0.83 (n = 6 cells) | 3 |
+CPA | 1.19 ± 0.09 (n = 55 events) | 4.0 ± 0.28 (n = 55 events) | 1.83 ± 0.22 (n = 6 cells) | 3 |
p | 0.55 | 0.58 | 0.0033 | |
Ip3r2 involvement | ||||
Global waves | ||||
WT littermate | 2.84 ± 0.13 (n = 25 waves, 273 locations) | 6.15 ± 0.2 (n = 25 waves, 273 locations) | 0.46 ± 0.15 (n = 11 cells) | 4 |
Ip3r2-/- | - (n = 0 waves) | - (n = 0 waves) | 0 ± 0 (n = 15 cells) | 4 |
p | ND | ND | 0.00016 | |
Local waves | ||||
WT littermate | 2.10 ± 0.08 (n = 122 waves, 241 locations) | 4.11 ± 0.13 (n = 122 waves, 241 locations) | 2.27 ± 0.34 (n = 11 cells) | 4 |
Ip3r2-/- | 1.35 ± 0.21 (n = 8 waves, 20 locations) | 5.53 ± 0.99 (n = 8 waves, 20 locations) | 0.02 ± 0.02 (n = 15 cells) | 4 |
p | 0.003 | 0.19 | 0.00001 | |
Microdomains | ||||
WT littermate | 1.36 ± 0.04 (n = 394 events) | 3.21 ± 0.09 (n = 394 events) | 7.20 ± 1.0 (n = 11 cells) | 4 |
Ip3r2-/- | 1.07 ± 0.04 (n = 310 events) | 3.63 ± 0.15 (n = 310 events) | 4.0 ± 0.6 (n = 15 cells) | 4 |
p | <0.00001 | 0.25 | 0.0003 |
-
CPA was used at 20 μm. TTX was used at 0.5 μm. Statistical tests were paired Student's t test for the CPA and TTX experiments and unpaired Student's t test or nonparametric Mann-Whitney tests as appropriate for the experiments with Ip3r2-/- mice. ND means not determined because there was not enough data to permit the comparison. Precise p-values are indicated. Significance was declared at p < 0.05. The experiments are described in the text.