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Article: Dysfunctional Calcium and Glutamate Signaling in Striatal Astrocytes from Huntington's Disease Model Mice.

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Jiang R; Diaz-Castro B; Looger LL; Khakh BS
J. Neurosci., 2016


Table 1.

Properties of Ca2+ global waves, local waves, and microdomains measured in striatal astrocytes in situ with GCaMP3

Peak δF/F T0.5 (s) Frequency (min-1) No. of mice
Basic properties
    Global waves
        Control 1.81 ± 0.08 (n = 26 waves, 189 locations) 4.94 ± 0.16 (n = 26 waves, 189 locations) 0.87 ± 0.22 (n = 6 cells) 4
        +TTX 1.66 ± 0.07 (n = 34 waves, 244 locations) 4.47 ± 0.13 (n = 34 waves, 244 locations) 1.13 ± 0.31 (n = 6 cells) 4
        p 0.07 0.02 0.136
    Local waves
        Control 1.72 ± 0.09 (n = 34 waves, 103 locations) 4.22 ± 0.19 (n = 34 waves, 103 locations) 1.13 ± 0.30 (n = 6 cells) 4
        +TTX 1.64 ± 0.08 (n = 39 waves, 106 locations) 4.14 ± 0.22 (n = 39 waves, 106 locations) 1.17 ± 0.08 (n = 6 cells) 4
        p 0.558 0.5 0.58
    Microdomains
        Control 1.29 ± 0.07 (n = 145 events) 3.01 ± 0.16 (n = 145 events) 4.83 ± 0.99 (n = 6 cells) 4
        +TTX 1.20 ± 0.05 (n = 151 events) 2.98 ± 0.11 (n = 151 events) 5.03 ± 1.04 (n = 6 cells) 4
        p 0.40 0.16 0.713
Store depletion
    Global waves
        Control 1.77 ± 0.07 (n = 23 waves, 161 locations) 5.77 ± 0.37 (n = 23 waves, 161 locations) 0.77 ± 0.18 (n = 6 cells) 3
        +CPA - (n = 0 waves) - (n = 0 waves) 0 ± 0 3
        p ND ND 0.0084
    Local waves
        Control 1.60 ± 0.06 (n = 50 waves, 103 locations) 5.15 ± 0.34 (n = 50 waves, 103 locations) 1.67 ± 0.22 (n = 6 cells) 3
        +CPA 1.60 ± 0.09 (n = 3 waves, 4 locations) 6.78 ± 1.14 (n = 3 waves, 4 locations) 0.1 ± 0.07 (n = 6 cells) 3
        p 0.98 0.10 0.0011
    Microdomains
        Control 1.14 ± 0.03 (n = 189 events) 4.0 ± 0.21 (n = 189 events) 6.3 ± 0.83 (n = 6 cells) 3
        +CPA 1.19 ± 0.09 (n = 55 events) 4.0 ± 0.28 (n = 55 events) 1.83 ± 0.22 (n = 6 cells) 3
        p 0.55 0.58 0.0033
Ip3r2 involvement
    Global waves
        WT littermate 2.84 ± 0.13 (n = 25 waves, 273 locations) 6.15 ± 0.2 (n = 25 waves, 273 locations) 0.46 ± 0.15 (n = 11 cells) 4
        Ip3r2-/- - (n = 0 waves) - (n = 0 waves) 0 ± 0 (n = 15 cells) 4
        p ND ND 0.00016
    Local waves
        WT littermate 2.10 ± 0.08 (n = 122 waves, 241 locations) 4.11 ± 0.13 (n = 122 waves, 241 locations) 2.27 ± 0.34 (n = 11 cells) 4
        Ip3r2-/- 1.35 ± 0.21 (n = 8 waves, 20 locations) 5.53 ± 0.99 (n = 8 waves, 20 locations) 0.02 ± 0.02 (n = 15 cells) 4
        p 0.003 0.19 0.00001
    Microdomains
        WT littermate 1.36 ± 0.04 (n = 394 events) 3.21 ± 0.09 (n = 394 events) 7.20 ± 1.0 (n = 11 cells) 4
        Ip3r2-/- 1.07 ± 0.04 (n = 310 events) 3.63 ± 0.15 (n = 310 events) 4.0 ± 0.6 (n = 15 cells) 4
        p <0.00001 0.25 0.0003
  • CPA was used at 20 μm. TTX was used at 0.5 μm. Statistical tests were paired Student's t test for the CPA and TTX experiments and unpaired Student's t test or nonparametric Mann-Whitney tests as appropriate for the experiments with Ip3r2-/- mice. ND means not determined because there was not enough data to permit the comparison. Precise p-values are indicated. Significance was declared at p < 0.05. The experiments are described in the text.


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