TABLE 2.Temperature effects on [Ca2+]i and the mAHP in response to a single AP
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| RT | 32–34°C | Q10 | |
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| Soma | |||
| [Ca2+]i amplitude, nM | 27 ± 13 (13) | 23 ± 14 (15) | 0.83 ± 0.63 |
| τdecay of [Ca2+]i | 1703 ± 855 (13) * | 479 ± 294 (15) | 0.21 ± 0.17 |
| Integral of [Ca2+]i, nM*s | 9.4 ± 5.1 (15) | 7.0 ± 5.1 (15) | 0.71 ± 0.64 |
| Apical dendrite | |||
| [Ca2+]i amplitude, nM | 81 + 42 (7) | 70 + 30 (15) | 0.63 + 0.19 |
| τdecay of [Ca2+]i | 337 + 113 (7) | 212 + 83 (7) | 0.69 + 0.21 |
| Integral of [Ca2+]i, nM*s | 16.5 + 8.9 (7) | 15.6 + 3.0 (7) | 1.06 + 0.48 |
| mAHP | |||
| Amplitude, mV | 5.3 ± 1.6 (20) | 4.7 ± 2.0 (20) | 0.91 ± 0.43 |
| TTP, ms | 60 ± 26 (20) * | 36 ± 15 (20) | 0.54 ± 0.32 |
| τrise | 18 ± 7 (20) * | 13 ± 5 (20) | 0.79 ± 0.4 |
| τfall | 164 ± 59 (20) * | 122 ± 66 (20) | 0.8 + 0.5 |
| ImAHP | |||
| Amplitude, pA | 65 ± 30 (20) | 83 ± 50 (27) | 1.57 ± 1.24 |
| TTP, ms * | 19 ± 6 (20) | 14 ± 5 (18) | 0.73 ± 0.48 |
| τdecay* | 94 ± 67 (20) | 67 ± 33 (27) | 0.59 ± 0.51 |
Means ± SD with number of cells in parentheses [Ca2+]i determined from dF/F using Eq. 1 in METHODS. [Ca2+]i amplitude, change from resting [Ca2+]i, mAHP, medium afterhyperpolarization; TTP, time to peak; τ, time constant;
* , significant difference between temperatures (P < 0.05, unpaired t-test). RT (room temperature) = 23 ± 1 °C. ImAHP was elicited with a 30-ms step to +10 mV.