Table 2. The effect of TNFα on passive membrane properties of GAD67+ GABAergic neurons in spinal lamina II
| Neurons recorded with normal pipette solution | Neurons recorded with SB202190 in pipette | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tonic firing | Initial bursting | Delayed firing | Tonic firing | |||||||
| Before | After | P | Before | After | Before | After | Before | After | P | |
| Values are mean ± SEM. P values: recorded before vs. after TNFα treatment in tonic firing neurons (paired t test). Cm, membrane capacitance; Rin, input resistance; RMP, resting membrane potential; CT, current threshold for action potential; AP, action potential. | ||||||||||
| Cm (pF) | 48.3 ± 2.9 | 44.7 ± 3.6 | 0.07 | 44.3 ± 13.3 | 44.8 ± 13.1 | 58.1 | 58.6 | 51.7 ± 3.5 | 49.5 ± 3.6 | 0.10 |
| Rin (MΩ) | 461.3 ± 40.9 | 272.3 ± 56.4 | < 0.01 | 366.0 ± 143.7 | 441.4 ± 167.0 | 426.7 | 118.3 | 370.4 ± 36.1 | 332.4 ± 31.2 | 0.06 |
| RMP (mV) | −51.6 ± 1.5 | −61.8 ± 1.6 | <0.01 | −51.0 ± 3.8 | −54.3 ± 3.5 | −65.0 | −75.0 | −51.9 ± 1.8 | −52.6 ± 1.9 | 0.39 |
| CT (pA) | 23.3 ± 1.9 | 65.0 ± 13.0 | 0.01 | 33.3 ± 13.3 | 26.7 ± 6.7 | 20.0 | 100.0 | 28.6 ± 3.5 | 35.7 ± 5.6 | 0.19 |
| N | 12 | 12 | 3 | 3 | 1 | 1 | 14 | 14 | ||