Table 4.
Cholinergic suppression of Schaffer–collateral EPSPs in hippocampal CA1 pyramidal neurons expressing different mAChR subunits
| Genotype | n | Baseline EPSP Amplitude, mV | EPSP in Carbachol, mV | EPSP After Wash, mV | Percentage Change in EPSP Amplitude (10 μM CCH) |
|---|---|---|---|---|---|
| Wild-type (WT) | 9 | 4.0 ± 0.8 | 1.5 ± 0.3 | 5.0 ± 1.4 | −61 ± 5% |
| Wild-type (PTX) | 6 | 4.8 ± 1.3 | 1.5 ± 0.2 | 3.6 ± 0.5 | −57 ± 10% |
| M1 KO | 9 | 5.9 ± 0.4 | 3.5 ± 0.5 | 6.1 ± 0.4 | −42 ± 6% |
| M2 KO | 7 | 5.1 ± 0.7 | 2.1 ± 0.4 | 4.6 ± 0.9 | −60 ± 4% |
| M4 KO | 13 | 4.7 ± 0.4 | 4.2 ± 0.5 | 4.8 ± 0.5 | −12 ± 8%* |
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Values are means ± SE. Cholinergic suppression of Schaffer–collateral EPSPs by bath-applied carbachol (10 μM for 5 min) in CA1 pyramidal neurons. Changes in EPSP amplitude were measured at the end of carbachol treatment and relative to values measured in baseline conditions. Numbers in boldface type indicate P < 0.05 for carbachol-induced effects within individual cell types (repeated-measures ANOVA). Asterisks indicate P < 0.01 when compared with results in wild-type neurons (one-way ANOVA with post-tests).