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Article: GAD67-GFP+ neurons in the Nucleus of Roller: a possible source of inhibitory input to hypoglossal motoneurons. I. Morphology and firing properties.

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van Brederode JF; Yanagawa Y; Berger AJ
J. Neurophysiol., 2011


Table 1

Intrinsic membrane properties of GAD67-GFP+ interneurons and hypoglossal motoneurons

Cell Type n Rn, mΩ Tau, ms C, pF Sag-ratio Vrest
A. Passive properties
Interneurons
    Tonic, adapting 17 888 ± 130 47 ± 5 62 ± 6 1.17 ± 0.05 −69.0 ± 2.1
    Tonic, nonadapting 10 788 ± 146 57 ± 12 50 ± 7 1.30 ± 0.09 −69.3 ± 2.0
    Tonic, accelerating 9 808 ± 81 47 ± 5 62 ± 7 1.13 ± 0.03 −72.1 ± 2.7
    Phasic 7 375 ± 68† 15 ± 3† 42 ± 6 1.10 ± 0.02 −68.3 ± 2.2
HMs 7 57 ± 130* 6 ± 1* 117 ± 28* 1.31 ± 0.08 −75.4 ± 1.7
    ANOVA, P value <0.05 <0.05 <0.05 >0.05 >0.05
Cell Type n APth, mV APh, ms APhw, mV AHP, mV tAHP, ms
B. Action potential properties
Interneurons
    Tonic, adapting 17 −51.6 ± 2.1 56.0 ± 4.0 1.76 ± 0.14 −20.6 ± 0.8 44.5 ± 5.93
    Tonic, nonadapting 10 −54.0 ± 1.4 58.7 ± 3.3 1.28 ± 0.10 −22.3 ± 1.1 33.6 ± 2.7
    Tonic, accelerating 9 −50.3 ± 2.5 59.3 ± 5.1 1.15 ± 0.08 −23.1 ± 2.1 28.0 ± 3.6
    Phasic 7 −52.2 ± 1.3 50.7 ± 2.6 1.39 ± 0.17 −17.1 ± 2.0† 11.0 ± 1.8†
HMs 7 −54.3 ± 2.2 62.3 ± 4.9 0.81 ± 0.05* −16.2 ± 1.4* 9.5 ± 3.1*
    ANOVA, P value >0.05 >0.05 <0.05 <0.05 <0.05
Cell Type n Adap-ratio Ith, pA fth, Hz fI slope, Hz fL slope, Hz
C. Firing properties
Interneurons
    Tonic, adapting 17 1.31 ± 0.04 22.5 ± 4.2 4.4 ± 0.5 9.5 ± 1.7 4.2 ± 0.8
    Tonic, nonadapting 10 1.01 ± 0.02 11.3 ± 1.7 5.0 ± 0.5 4.1 ± 0.6 2.8 ± 0.5
    Tonic, accelerating 9 0.83 ± 0.03 16.3 ± 3.4 4.9 ± 0.7 4.1 ± 0.3 3.6 ± 0.4
    Phasic 7 NA 42.9 ± 9.2† 7.8 ± 2.3† 12.8 ± 3.4 8.0 ± 1.9
HMs 7 0.86 ± 0.10* 367.9 ± 77.8* 8.8 ± 1.2* 15.3 ± 3.7* 17.4 ± 3.3*
    ANOVA, P value <0.05 <0.05 <0.05 <0.05 <0.05

  • Values are means ± SE. Rn, input resistance; tau, membrane time constant; C, total cell capacitance; Sag, sag-ratio calculated from: Vpeak/Vss (see methods); Vrest, resting membrane potential; APth, action potential threshold; APh, action potential height; APhw, action potential half-width; AHP, magnitude of the maximum spike afterhyperpolarization; tAHP, time to the peak spike afterhyperpolarization; Adap-ratio, frequency adaptation ratio calculated from the firing response to square current pulses (see methods); Ith, minimum or threshold current amplitude needed to elicit repetitive firing; fth, threshold frequency for repetitive firing; f1slope, slope of the relationship between the instantaneous frequency of the first spike interval in the train and the amplitude of the injected current pulse normalized by expressing current as a multiple of the threshold current (threshold current = 1); fL slope, slope of the normalized f–I relationship for the last spike interval in the train. ANOVA was performed between all five groups of cells. Significant differences between groups are discussed in the results.

  • ↵* Significant difference between interneurons and HMs (t-test, P < 0.05).

  • ↵† Significant difference between phasic and tonic interneurons (Tukey test, P < 0.05).


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Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Hypoglossal nucleus motor neuron input resistance (mΩ) 57.0 ± 130.0 -- Data Table
Hypoglossal nucleus motor neuron membrane time constant (ms) 6.0 ± 1.0 -- Data Table
Hypoglossal nucleus motor neuron sag ratio 1.31 ± 0.08 -- Data Table
Hypoglossal nucleus motor neuron resting membrane potential -75.4 ± 1.7 -- Data Table