Article: A spontaneous mutation involving Kcnq2 (Kv7.2) reduces M-current density and spike frequency adaptation in mouse CA1 neurons.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Otto JF; Yang Y; Frankel WN; White HS; Wilcox KS J. Neurosci., 2006
Membrane property | B6 | n | Szt1 | n |
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Resting membrane potential (mV) | −60.1 ± 0.4 | 19 | −59.7 ± 0.4 | 19 |
Holding current at −20 mV (pA) | 261 ± 22 | 28 | 280 ± 31 | 31 |
Input resistance (MΩ) | 111 ± 4 | 38 | 105 ± 4 | 40 |
Series resistance (MΩ) | 13.2 ± 0.5 | 38 | 13.4 ± 0.5 | 40 |
Spike half-width (ms) | 1.52 ± 0.04 | 19 | 1.53 ± 0.04 | 18 |
Spike threshold (mV) | −46.2 ± 0.7 | 19 | −45.0 ± 0.7 | 18 |
IK(M) deactivation kinetics (τ in ms) | 157 ± 13 | 17 | 140 ± 15 | 17 |
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None of the parameters measured differed between CA1 neurons in slices prepared from B6 and Szt1 mice (p > 0.5, ANOVA). Resting membrane potential, input resistance, and series resistance were taken from values reported by SealTest and MultiClamp Commander functions. Spike half-width and spike threshold values were calculated from the first action potential that was generated in response to the lowest depolarizing current injection. > values were calculated by fitting the IK(M) deactivation phase with a single-exponential equation.