AboutNeuron TypesElectrophysiology PropertiesArticlesFAQsData/APIGet Involved

Article: Impaired action potential initiation in GABAergic interneurons causes hyperexcitable networks in an epileptic mouse model carrying a human Na(V)1.1 mutation.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Hedrich UB; Liautard C; Kirschenbaum D; Pofahl M; Lavigne J; Liu Y; Theiss S; Slotta J; Escayg A; Dihné M; Beck H; Mantegazza M; Lerche H
J. Neurosci., 2014


Table 1.

Membrane properties of different neurons recorded in brain slices of WT and mutant animals

Animals Vm (mV) Rin (MΩ) AP threshold for eliciting 1 AP (mV) 1 AP width (ms) Rheobase (pA)* Cells (n)
Nucleus reticularis, burster
    WT -68.6 ± 3.5 181.4 ± 14.9 -50.1 ± 4.1 0.65 ± 0.03 47.6 ± 4.5 23
    Scn1aRH/+ -69.6 ± 3.6 156.8 ± 13.0 -49.1 ± 2.5 0.61 ± 0.02 54.1 ± 4.3 37
    Scn1aRH/RH -69.9 ± 0.8 225.5 ± 17.9 -52.0 ± 0.8 0.64 ± 0.02 57.5 ± 5.8 10
Nucleus reticularis, non-burster
    WT -72.3 ± 1.7 303.0 ± 47.9 -53.1 ± 0.9 1.04 ± 0.07 59.3 ± 7.4 7
    Scn1aRH/+ -72.3 ± 1.5 306.2 ± 69.5 -55.0 ± 5.3 1.02 ± 0.08 79.3 ± 10.9 7
VB, relay neuron
    WT -72.1 ± 1.0 104.5 ± 4.1 -57.3 ± 1.8 1.03 ± 0.02 97.7 ± 8.6 11
    Scn1aRH/+ -70.9 ± 1.8 134.75 ± 16.2 -55.3 ± 1.3 1.01 ± 0.03 87.5 ± 11.7 13
    Scn1aRH/RH -69.3 ± 1.4 153.82 ± 17.48 -52.6 ± 1.0 1.02 ± 0.04 87.5 ± 12.5 6
Cortex, inhibitory neurons
    WT -70.1 ± 1.4 200.1 ± 16.8 -54.1 ± 1.8 0.44 ± 0.04 92.9 ± 13.0 7
    Scn1aRH/+ -69.9 ± 1.5 196.7 ± 14.6 -52.4 ± 1.3 0.54 ± 0.03 105.0 ± 16.2 9
Cortex, pyramidal cells
    WT -72.9 ± 1.1 140.7 ± 11.2 -54.7 ± 1.8 1.02 ± 0.05 107.8 ± 9.9 17
    Scn1aRH/+ -74.1 ± 1.0 152.0 ± 8.9 -57.2 ± 1.5 1.03 ± 0.04 101.0 ± 7.8 26
Hippocampus, Stratum oriens
    WT -74.5 ± 5.1 174.9 ± 11.4 -54.4 ± 1.0 0.57 ± 0.07 50.0 ± 9.5 7
    Scn1aRH/+ -73.4 ± 4.8 163.7 ± 16.9 -53.1 ± 1.9 0.57 ± 0.05 79.6 ± 16.8 11
    Scn1aRH/RH -69.3 ± 1.1 206.4 ± 14.2 -52.8 ± 1.1 0.58 ± 0.03 64.2 ± 7.7 6
Hippocampus, Stratum pyramidale
    WT -74.8 ± 1.2 226.2 ± 14.0 -53.4 ± 1.2 1.04 ± 0.02 50.0 ± 5.9 15
    Scn1aRH/+ -71.0 ± 1.2 190.8 ± 19.1 -55.8 ± 1.6 1.06 ± 0.04 55.6 ± 12.3 14
    Scn1aRH/RH -70.7 ± 2.1 183.4 ± 16.9 -54.0 ± 1.7 1.08 ± 0.02 43.8 ± 6.3 8
  • Data are presented as means ± SEM; Mann-Whitney rank-sum test and ANOVA on ranks with Dunn's post hoc test.

  • *Current injection of 800 ms. Vm, resting membrane potential; Rin, input resistance.


Report miscurated data

Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Thalamic reticular nucleus cell thalamic reticular nucleus bursting GABAergic inhibitory neuron input resistance (MΩ) 181.4 ± 14.9 (23) 181.4 (MΩ) Data Table
Neocortex pyramidal cell layer 5-6 primary somatosensory cortex layer 5 pyramidal cell resting membrane potential (mV) -72.9 ± 1.1 (17) -72.9 (mV) Data Table
Neocortex pyramidal cell layer 5-6 primary somatosensory cortex layer 5 pyramidal cell input resistance (MΩ) 140.7 ± 11.2 (17) 140.7 (MΩ) Data Table
Neocortex pyramidal cell layer 5-6 primary somatosensory cortex layer 5 pyramidal cell spike threshold (mV) -54.7 ± 1.8 (17) -54.7 (mV) Data Table
Thalamic reticular nucleus cell thalamic reticular nucleus bursting GABAergic inhibitory neuron spike threshold (mV) -50.1 ± 4.1 (23) -50.1 (mV) Data Table
Neocortex pyramidal cell layer 5-6 primary somatosensory cortex layer 5 pyramidal cell spike width (ms) 1.02 ± 0.05 (17) 1.02 (ms) Data Table
Neocortex pyramidal cell layer 5-6 primary somatosensory cortex layer 5 pyramidal cell rheobase (pA) 107.8 ± 9.9 (17) 107.8 (pA) Data Table
Thalamic reticular nucleus cell thalamic reticular nucleus bursting GABAergic inhibitory neuron spike width (ms) 0.65 ± 0.03 (23) 0.65 (ms) Data Table
Hippocampus CA1 basket cell Hippocampus CA1 Stratum Oriens fast-spiking inhibitory neuron resting membrane potential (mV) -74.5 ± 5.1 (7) -74.5 (mV) Data Table
Hippocampus CA1 basket cell Hippocampus CA1 Stratum Oriens fast-spiking inhibitory neuron input resistance (MΩ) 174.9 ± 11.4 (7) 174.9 (MΩ) Data Table
Thalamic reticular nucleus cell thalamic reticular nucleus bursting GABAergic inhibitory neuron rheobase (pA) 47.6 ± 4.5 (23) 47.6 (pA) Data Table
Hippocampus CA1 basket cell Hippocampus CA1 Stratum Oriens fast-spiking inhibitory neuron spike threshold (mV) -54.4 ± 1.0 (7) -54.4 (mV) Data Table
Hippocampus CA1 basket cell Hippocampus CA1 Stratum Oriens fast-spiking inhibitory neuron spike width (ms) 0.57 ± 0.07 (7) 0.57 (ms) Data Table
Hippocampus CA1 basket cell Hippocampus CA1 Stratum Oriens fast-spiking inhibitory neuron rheobase (pA) 50.0 ± 9.5 (7) 50.0 (pA) Data Table
Hippocampus CA1 pyramidal cell Hippocampus CA1 stratum pyramidale pyramidal cell resting membrane potential (mV) -74.8 ± 1.2 (15) -74.8 (mV) Data Table
Hippocampus CA1 pyramidal cell Hippocampus CA1 stratum pyramidale pyramidal cell input resistance (MΩ) 226.2 ± 14.0 (15) 226.2 (MΩ) Data Table
Hippocampus CA1 pyramidal cell Hippocampus CA1 stratum pyramidale pyramidal cell spike threshold (mV) -53.4 ± 1.2 (15) -53.4 (mV) Data Table
Hippocampus CA1 pyramidal cell Hippocampus CA1 stratum pyramidale pyramidal cell spike width (ms) 1.04 ± 0.02 (15) 1.04 (ms) Data Table
Hippocampus CA1 pyramidal cell Hippocampus CA1 stratum pyramidale pyramidal cell rheobase (pA) 50.0 ± 5.9 (15) 50.0 (pA) Data Table
Thalamic reticular nucleus cell Thalamic reticular nucleus non-bursting GABAergic inhibitory neuron resting membrane potential (mV) -72.3 ± 1.7 (7) -72.3 (mV) Data Table
Thalamic reticular nucleus cell Thalamic reticular nucleus non-bursting GABAergic inhibitory neuron input resistance (MΩ) 303.0 ± 47.9 (7) 303.0 (MΩ) Data Table
Thalamic reticular nucleus cell Thalamic reticular nucleus non-bursting GABAergic inhibitory neuron spike threshold (mV) -53.1 ± 0.9 (7) -53.1 (mV) Data Table
Thalamic reticular nucleus cell Thalamic reticular nucleus non-bursting GABAergic inhibitory neuron spike width (ms) 1.04 ± 0.07 (7) 1.04 (ms) Data Table
Thalamic reticular nucleus cell Thalamic reticular nucleus non-bursting GABAergic inhibitory neuron rheobase (pA) 59.3 ± 7.4 (7) 59.3 (pA) Data Table
Thalamus relay cell Ventrobasal thalamocortical excitatory relay neuron resting membrane potential (mV) -72.1 ± 1.0 (11) -72.1 (mV) Data Table
Thalamus relay cell Ventrobasal thalamocortical excitatory relay neuron input resistance (MΩ) 104.5 ± 4.1 (11) 104.5 (MΩ) Data Table
Thalamus relay cell Ventrobasal thalamocortical excitatory relay neuron spike threshold (mV) -57.3 ± 1.8 (11) -57.3 (mV) Data Table
Thalamus relay cell Ventrobasal thalamocortical excitatory relay neuron spike width (ms) 1.03 ± 0.02 (11) 1.03 (ms) Data Table
Thalamus relay cell Ventrobasal thalamocortical excitatory relay neuron rheobase (pA) 97.7 ± 8.6 (11) 97.7 (pA) Data Table
Neocortex basket cell Primary somatosensory cortex layer 4 fast-spiking inhibitory neuron resting membrane potential (mV) -70.1 ± 1.4 (7) -70.1 (mV) Data Table
Neocortex basket cell Primary somatosensory cortex layer 4 fast-spiking inhibitory neuron input resistance (MΩ) 200.1 ± 16.8 (7) 200.1 (MΩ) Data Table
Neocortex basket cell Primary somatosensory cortex layer 4 fast-spiking inhibitory neuron spike threshold (mV) -54.1 ± 1.8 (7) -54.1 (mV) Data Table
Neocortex basket cell Primary somatosensory cortex layer 4 fast-spiking inhibitory neuron spike width (ms) 0.44 ± 0.04 (7) 0.44 (ms) Data Table
Thalamic reticular nucleus cell thalamic reticular nucleus bursting GABAergic inhibitory neuron resting membrane potential (mV) -68.6 ± 3.5 (23) -68.6 (mV) Data Table
Neocortex basket cell Primary somatosensory cortex layer 4 fast-spiking inhibitory neuron rheobase (pA) 92.9 ± 13.0 (7) 92.9 (pA) Data Table