Comparison of properties of neurons in ICR, HCN1+/+, and HCN1−/− mice
| Octopus | T stellate | Bushy | |||||||
|---|---|---|---|---|---|---|---|---|---|
| ICR | HCN1+/+ | HCN1−/− | ICR | HCN1+/+ | HCN1−/− | ICR | HCN1+/+ | HCN1−/− | |
| Input resistance (MΩ) | 6 ± 2 (10)* | 14 ± 2 (8) | 19 ± 3 (19)† | 74 ± 11 (12)* | 126 ± 11 (7) | 160 ± 27 (9)† | 67 ± 17 (36) | 68 ± 22 (11) | 99 ± 24 (12)† |
| Time constant (ms) | 0.2 ± 0.1 (9) | 0.3 ± 0.2 (8) | 0.5 ± 0.1 (5)† | 6.0 ± 2.7 (12) | 6.8 ± 1 (6) | 7.5 ± 3.2 (8) | 1.4 ± 0.5(18) | 1.2 ± 0.3(10) | 2.6 ± 1.1 (11)† |
| Resting potential (mV) | −64.5 ± 1.3 (8) | −62 ± 2 (13) | −64 ± 1 (5) | −64.1 ± 0.8 (10) | −64 ± 2.4 (8) | −65.8 ± 1.3(13) | −64.4 ± 0.6 (9) | −63 ± 1 (8) | −66 ± 1.3 (6) |
| Activation of gh: τf −57 → −107 mV (ms) | 23 ± 4 (7) 81% | 100 ± 19 (6) 63% | 270 ± 34 (5) 60% | 140 ± 35 (5) 45% | 162 ± 54 (5) 57% | 460 ± 84 (5) 44% | 94 ± 17 (7) 54% | 116 ± 38 (9) 56% | 490 ± 94 (8) 45% |
| Activation of gh: τs −57 → −107 mV (ms) | 99 ± 23 (7) 19% | 1131 ± 396 (6) 37% | 2120 ± 760 (5) 40% | 770 ± 200 (5) 55% | 1053 ± 404 (5) 43% | 2430 ± 480 (5) 56% | 630 ± 130 (7) 46% | 945 ± 215 (9) 44% | 2790 ± 670 (8) 55% |
| Deactivation of gh: τ −107 → −57 mV (ms) | 107 ± 21 (7)* | 307 ± 79 (6) | 430 ± 41 (5)† | 275 ± 78 (5) | 313 ± 74 (5) | 440 ± 94 (5)† | 196 ± 34 (7)* | 329 ± 54 (9) | 437 ± 117 (8)† |
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Summary of the differences in the properties of neurons in mice of different strains. Numbers are given as means ± SD, with the number of cells from which measurements were made given in parentheses.
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↵* Significance of difference, P < 0.05, between ICR and hyperpolarization-activated cyclic nucleotide-gated 1 channel wild-type control (HCN1+/+).
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↵† Significance of difference, P < 0.05, between mice that lack the HCN1 α subunit (HCN1−/−) and HCN1+/+. gh, Hyperpolarization-activated, mixed-cation conductance; τf and τs, fast and slow time constants, respectively.