Membrane properties of CA1 pyramidal neurons under whole-cell current clamp
| rp(mV) | RIN(MΩ) | APAMP(mV) | APTH(mV) | APDUR (msec) | τ (msec) | n | |
|---|---|---|---|---|---|---|---|
| K-gluconate1_a | −65 ± 0.1 | 149 ± 3 | 92 ± 1 | −47 ± 0.3 | 1.6 ± 0.01 | 20.7 ± 0.4 | 188 |
| Cs-gluconate1_b | −59 ± 0.7 | 183 ± 12 | – | – | – | 23.6 ± 1.8 | 11 |
| KClc | −65 ± 0.4 | 173 ± 24 | 92 ± 5 | −48 ± 1.3 | 1.8 ± 0.06 | 22.8 ± 3.0 | 8 |
| BAPTAd | −67 ± 1.9 | 164 ± 47 | 88 ± 2 | −45 ± 1.4 | 2.4 ± 0.15 | 21.6 ± 1.1 | 4 |
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Abbreviations: rp, resting potential;R IN, input resistance;AP AMP, action potential amplitude;AP TH, action potential threshold;AP DUR, action potential duration; τ, tau.
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Intracellular solutions (in mm):
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↵F1_a 140 K-gluconate; 1.1 EGTA; 0.1 CaCl2; 10 HEPES; 2 Mg-ATP; 0.3 Na-GTP.
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↵F1_b 40 Cs-gluconate; 100 K-gluconate; 1.1 EGTA; 0.1 CaCl2; 10 HEPES; 2 Mg-ATP; 0.3 Na-GTP.
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c70 KCl; 70 K-gluconate; 1.1 EGTA; 0.1 CaCl2; 10 HEPES; 2 Mg-ATP; 0.3 Na-GTP.
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d100 K-gluconate; 10 K-BAPTA; 40 HEPES; 2 Mg-ATP; 0.3 Na-GTP.